LOCUS       sequence-352741-        2501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-pA7
FEATURES             Location/Qualifiers
     source          1..2501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     139..158
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(558..563,564..580,581..586))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(713..1301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(793..812)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1607..1623
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1607..1623
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1628..1647
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1628..1646
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1821..2501,1..510))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 gttgagcacc gccgccgcaa ggaatggtgc atgtaaggag atggcgccca acagtccccc
       61 ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
      121 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
      181 gccggtgatg ccggccacga tgcgtccggc gtagagaatc cacaggacgg gtgtggtcgc
      241 catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc
      301 aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag
      361 cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag
      421 gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag
      481 gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa
      541 ctgtgataaa ctaccgcatt aaagctaatc gatgataagc tgtcaaacat gagaattaat
      601 tcatgttctt tcctgcgtta tcccctgatt ctgtggataa cgcaggaaag aacatgtgag
      661 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      721 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      781 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      841 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      901 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      961 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1021 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1081 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1141 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1201 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1261 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1321 ctacggggtc tgacgctcag tggaacgacg cgtaactcac gttaagggat tttggtcatg
     1381 ggtggctcga ccagatatgg tctcaacttg caatcgctac aacttgatct ggggtgagtc
     1441 cgtgggcttg acagagaact ggaaggaggc gctgaaccgt ctcaagagcc tgtttgccac
     1501 caatattact ggctcgagac cagctttggt ctagaggagg catgatgata tatttttatc
     1561 ttgtgcaatg taacatcaga gattttgaga cacgggccag agctgccagg aaacagctat
     1621 gaccatgtaa tacgactcac tataggggat attttatttt ctcccaatca ggcttgatcc
     1681 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatcaagggt tggtttgcgc
     1741 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
     1801 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac
     1861 gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg
     1921 ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga agttaggctg
     1981 gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
     2041 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
     2101 aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc
     2161 atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag
     2221 gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg
     2281 ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg
     2341 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
     2401 cggaaggagc tgactgggtt gaaggctctc aagggcatgg gtcggcgctc tcccttatgc
     2461 gactcctgca ttaggaagca gcccagtagt aggttgaggc c
//
