LOCUS       sequence-352753-        8772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.C.HSV_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..134
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(135..203,204..995)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(353..372)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1166..1754
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1655..1674
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1908..1925
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2042..2063
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2078..2083,2084..2101,2102..2108)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2116..2132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2121..2143
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2140..2156
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2140..2156
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2175..2195
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2177..2195
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2223..2449
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2450..2630
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2677..2802
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3295..3528
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3713..3757
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4055..4303
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4055..4075
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4226..4245
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4322..4397
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4445..4562
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4702..5880
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4933..5871
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5828..5848
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5929..5938
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5977..6012
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6031..6063
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             6073..6780
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6218..6236)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6486..6505
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6850..7438
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6903..6923)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7321..7332)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7513..7746
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7818..7939
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7855..7874)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7909..7928
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7979..8114
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8134..8153)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8135..8153)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8163..8180)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8163..8179)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8172..8194)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8321..8749
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8408..8427)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8618..8639
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
       61 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      121 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      181 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
      241 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
      301 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
      361 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
      421 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
      481 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
      541 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
      601 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
      661 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
      721 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
      781 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
      841 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
      901 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
      961 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     1021 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1081 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1141 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1201 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1261 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     1321 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1381 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1441 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1501 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     1561 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     1621 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     1681 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     1741 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     1801 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     1861 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     1921 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     1981 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     2041 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     2101 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     2161 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     2221 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     2281 tgccttacaa ggagagaaaa agcaccgtgc atggcgattg gtggaagtaa ggtggtacga
     2341 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     2401 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     2461 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     2521 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     2581 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     2641 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     2701 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     2761 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     2821 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     2881 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     2941 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     3001 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     3061 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     3121 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     3181 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     3241 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     3301 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     3361 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     3421 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     3481 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     3541 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     3601 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     3661 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     3721 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     3781 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     3841 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     3901 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     3961 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatc
     4021 acgagactag tatcgagcgg ccgccccctt caccgagggc ctatttccca tgattccttc
     4081 atatttgcat atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa
     4141 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     4201 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     4261 cgatttcttg gctttatata tcttgtggaa aggacgaaac accgggtctt cgagaagacc
     4321 tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
     4381 tggcaccgag tcggtgcttt ttttaattct cgacctcgag acaaatggca gtattcatcc
     4441 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     4501 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     4561 ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag gcgataagct
     4621 ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
     4681 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc
     4741 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg
     4801 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     4861 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     4921 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
     4981 ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag
     5041 cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct
     5101 cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac
     5161 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct
     5221 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact
     5281 ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt
     5341 tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct
     5401 ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca
     5461 aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct
     5521 gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca
     5581 caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg
     5641 gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg
     5701 ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg
     5761 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg
     5821 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga
     5881 ggaattctgc agtcgatcga cggtaccgcg ggcccgggat ccacggtcgc caccatgggc
     5941 gacacctatc ggtacattgg aggaggcggg ggaggggagg accaggtgga ccccaggctg
     6001 atcgacggca agggaggagg cgggggaggg cagcccgagc tggcccccga ggaccccgag
     6061 gacggcatgc tgatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     6121 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     6181 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     6241 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     6301 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     6361 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     6421 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     6481 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     6541 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     6601 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     6661 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     6721 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     6781 aaacggccag ccgaggatat ggaggaagaa caagccttta aacgatctcg ataaagcggc
     6841 cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6901 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6961 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     7021 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     7081 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7141 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     7201 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     7261 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7321 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7381 ccgcatcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     7441 gggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7501 aaaagggggg actggaaggg ctaattcact cccaacgaag ataagatctg ctttttgctt
     7561 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7621 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7681 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7741 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7801 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7861 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7921 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     7981 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8041 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8101 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     8161 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     8221 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     8281 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     8341 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     8401 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     8461 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     8521 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     8581 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     8641 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     8701 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     8761 ttaacgctta ca
//
