LOCUS       sequence-352754-        8771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression of human PCAF in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Jin Q, Zhuang L, Lai B, Wang C, Li W, Dolan B, Lu Y, Wang Z, Zhao K,
            Peng W, Dent SY, Ge K
  TITLE     Gcn5 and PCAF negatively regulate interferon-beta production through
            HAT-independent inhibition of TBK1.
  JOURNAL   EMBO Rep. 2014 Nov;15(11):1192-201. doi: 10.15252/embr.201438990. 
            Epub 2014 Sep 30.
  PUBMED    25269644
REFERENCE   2  (bases 1 to 8771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embr.201438990"; journalName: "EMBO Rep"; date: "2014-11";
            volume: "15"; issue: "11"; pages: "1192-201"
COMMENT     Sequence Label: pMSCVpuro-PCAF
FEATURES             Location/Qualifiers
     source          1..8771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        3947..4446
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3982..4005)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4379..4398
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4467..5066
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4467..4486)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4963..4983
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5166..5680
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5842..5858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5866..5872,5873..5890,5891..5896))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5911..5932
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6049..6066)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6220..6808)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6300..6319)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6979..7770,7771..7839))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7602..7621
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7840..7944)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8012..8030
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     8190..8209
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8403..8425
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8553..8572
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatcttgg ccaccatgtc cgaggctggc
     1441 ggggccgggc cgggcggctg cggggcagga gccggggcag gggccgggcc cggggcgctg
     1501 cccccgcagc ctgcggcgct tccgcccgcg cccccgcagg gctccccctg cgccgctgcc
     1561 gccgggggct cgggcgcctg cggtccggcg acggcagtgg ctgcagcggg cacggccgaa
     1621 ggaccgggag gcggtggctc ggcccgaatc gccgtgaaga aagcgcaact acgctccgct
     1681 ccgcgggcca agaaactgga gaaactcgga gtgtactccg cctgcaaggc cgaggagtct
     1741 tgtaaatgta atggctggaa aaaccctaac ccctcaccca ctccccccag agccgacctg
     1801 cagcaaataa ttgtcagtct aacagaatcc tgtcggagtt gtagccatgc cctagctgct
     1861 catgtttccc acctggagaa tgtgtcagag gaagaaatga acagactcct gggaatagta
     1921 ttggatgtgg aatatctctt tacctgtgtc cacaaggaag aagatgcaga taccaaacaa
     1981 gtttatttct atctatttaa gctcttgaga aagtctattt tacaaagagg aaaacctgtg
     2041 gttgaaggct ctttggaaaa gaaaccccca tttgaaaaac ctagcattga acagggtgtg
     2101 aataactttg tgcagtacaa atttagtcac ctgccagcaa aagaaaggca aacaatagtt
     2161 gagttggcaa aaatgttcct aaaccgcatc aactattggc atctggaggc accatctcaa
     2221 cgaagactgc gatctcccaa tgatgatatt tctggataca aagagaacta cacaaggtgg
     2281 ctgtgttact gcaacgtgcc acagttctgc gacagtctac ctcggtacga aaccacacag
     2341 gtgtttggga gaacattgct tcgctcggtc ttcactgtta tgaggcgaca actcctggaa
     2401 caagcaagac aggaaaaaga taaactgcct cttgaaaaac gaactctaat cctcactcat
     2461 ttcccaaaat ttctgtccat gctagaagaa gaagtatata gtcaaaactc tcccatctgg
     2521 gatcaggatt ttctctcagc ctcttccaga accagccagc taggcatcca aacagttatc
     2581 aatccacctc ctgtggctgg gacaatttca tacaattcaa cctcatcttc ccttgagcag
     2641 ccaaacgcag ggagcagcag tcctgcctgc aaagcctctt ctggacttga ggcaaaccca
     2701 ggagaaaaga ggaaaatgac tgattctcat gttctggagg aggccaagaa accccgagtt
     2761 atgggggata ttccgatggg attaatcaac gaggttatgt ctaccatcac ggaccctgca
     2821 gcaatgcttg gaccagagac caattttctg tcagcacact cggccaggga tgaggcggca
     2881 aggttggaag agcgcagggg tgtaattgaa tttcacgtgg ttggcaattc cctcaaccag
     2941 aaaccaaaca agaagatcct gatgtggctg gttggcctac agaacgtttt ctcccaccag
     3001 ctgccccgaa tgccaaaaga atacatcaca cggctcgtct ttgacccgaa acacaaaacc
     3061 cttgctttaa ttaaagatgg ccgtgttatt ggtggtatct gtttccgtat gttcccatct
     3121 caaggattca cagagattgt cttctgtgct gtaacctcaa atgagcaagt caagggctat
     3181 ggaacacacc tgatgaatca tttgaaagaa tatcacataa agcatgacat cctgaacttc
     3241 ctcacatatg cagatgaata tgcaattgga tactttaaga aacagggttt ctccaaagaa
     3301 attaaaatac ctaaaaccaa atatgttggc tatatcaagg attatgaagg agccacttta
     3361 atgggatgtg agctaaatcc acggatcccg tacacagaat tttctgtcat cattaaaaag
     3421 cagaaggaga taattaaaaa actgattgaa agaaaacagg cacaaattcg aaaagtttac
     3481 cctggacttt catgttttaa agatggagtt cgacagattc ctatagaaag cattcctgga
     3541 attagagaga caggctggaa accgagtgga aaagagaaaa gtaaagagcc cagagaccct
     3601 gaccagcttt acagcacgct caagagcatc ctccagcagg tgaagagcca tcaaagcgct
     3661 tggcccttca tggaacctgt gaagagaaca gaagctccag gatattatga agttataagg
     3721 ttccccatgg atctgaaaac catgagtgaa cgcctcaaga ataggtacta cgtgtctaag
     3781 aaattattca tggcagactt acagcgagtc tttaccaatt gcaaagagta caacccccct
     3841 gagagtgaat actacaaatg tgccaatatc ctggagaaat tcttcttcag taaaattaag
     3901 gaagctggat taattgacaa gtgactcgag gttaacgaat tctaccgggt aggggaggcg
     3961 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
     4021 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
     4081 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
     4141 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
     4201 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
     4261 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
     4321 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
     4381 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
     4441 ctttcgacct gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     4501 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     4561 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     4621 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     4681 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     4741 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     4801 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     4861 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     4921 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     4981 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     5041 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
     5101 ggagcgcacg accccatgca tcgataaaat aaaagatttt atttagtctc cagaaaaagg
     5161 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
     5221 aggcatggaa aatacataac tgagaataga gaagttcaga tcaaggttag gaacagagag
     5281 acagcagaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     5341 ccaagaacag atggtcccca gatgcggtcc cgccctcagc agtttctaga gaaccatcag
     5401 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
     5461 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca
     5521 caacccctca ctcggcgcgc cagtcctccg atagactgcg tcgcccgggt acccgtgtat
     5581 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct
     5641 cctctgagtg attgactacc cgtcagcggg ggtctttcat gggtaacagt ttcttgaagt
     5701 tggagaacaa cattctgagg gtaggagtcg aatattaagt aatcctgact caattagcca
     5761 ctgttttgaa tccacatact ccaatactcc tgaaatagtt cattatggac agcgcagaaa
     5821 gagctgggga gaattgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     5881 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     5941 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6001 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6061 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6121 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6181 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6241 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6301 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6361 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6421 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6481 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6541 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6601 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6661 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6721 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6781 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6841 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6901 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6961 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7021 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7081 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7141 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7201 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7261 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7321 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7381 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7441 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7501 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7561 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7621 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7681 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7741 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7801 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7861 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7921 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     7981 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     8041 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     8101 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     8161 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     8221 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     8281 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     8341 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     8401 ttcccagtca cgacgttgta aaacgacggc gcaaggaatg gtgcatgcaa ggagatggcg
     8461 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
     8521 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     8581 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gcgattagtc
     8641 caatttgtta aagacaggat atcagtggtc caggctctag ttttgactca acaatatcac
     8701 cagctgaagc ctatagagta cgagccatag ataaaataaa agattttatt tagtctccag
     8761 aaaaaggggg g
//
