LOCUS       sequence-352756-        8784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.C.Ollas_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        76..180
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(181..249,250..1041)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(399..418)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1212..1800
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1701..1720
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1954..1971
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2088..2109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2124..2129,2130..2147,2148..2154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2162..2178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2167..2189
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2186..2202
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2186..2202
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2221..2241
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2223..2241
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2269..2495
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2496..2676
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2723..2848
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3341..3574
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3759..3803
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4101..4349
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4101..4121
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4272..4291
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4368..4443
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4491..4608
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4748..5926
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4979..5917
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5874..5894
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5975..5984
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6023..6058
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6077..6118
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             6131..6838
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6276..6294)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6544..6563
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6908..7496
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6961..6981)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7379..7390)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7571..7804
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7876..7997
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7913..7932)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7967..7986
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8037..8172
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8192..8211)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8193..8211)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8221..8238)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8221..8237)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8230..8252)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8379..8784,1..50)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8466..8485)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8676..8697
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
       61 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      121 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      181 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      241 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      301 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      361 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      421 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      481 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      541 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      601 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      661 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      721 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      781 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      841 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      901 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      961 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     1021 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     1081 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     1141 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1201 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1261 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1321 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1381 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1441 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1501 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1561 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1621 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1681 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1741 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1801 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1861 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     1921 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     1981 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     2041 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     2101 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     2161 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     2221 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     2281 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     2341 agaaaaagca ccgtgcatgg cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     2401 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     2461 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     2521 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     2581 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     2641 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     2701 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     2761 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     2821 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     2881 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     2941 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     3001 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     3061 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     3121 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     3181 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     3241 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     3301 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     3361 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     3421 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     3481 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     3541 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     3601 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     3661 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     3721 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     3781 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     3841 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     3901 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     3961 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     4021 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagtatc
     4081 gagcggccgc ccccttcacc gagggcctat ttcccatgat tccttcatat ttgcatatac
     4141 gatacaaggc tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag
     4201 tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat
     4261 gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt
     4321 tatatatctt gtggaaagga cgaaacaccg ggtcttcgag aagacctgtt ttagagctag
     4381 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     4441 tgcttttttt aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga
     4501 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     4561 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     4621 agggacagca gagatccact ttggccgcgg ctcgaggcga taagctttgc aaagatggat
     4681 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
     4741 gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     4801 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     4861 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     4921 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     4981 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
     5041 ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
     5101 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga
     5161 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
     5221 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
     5281 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
     5341 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     5401 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     5461 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     5521 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
     5581 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
     5641 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     5701 cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     5761 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     5821 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     5881 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa ttctgcagtc
     5941 gatcgacggt accgcgggcc cgggatccac ggtcgccacc atgggcgaca cctatcggta
     6001 cattggagga ggcgggggag gggaggacca ggtggacccc aggctgatcg acggcaaggg
     6061 aggaggcggg ggagggagcg gcttcgccaa cgagctgggc cccaggctga tgggcaagtg
     6121 cggcatgctg atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     6181 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     6241 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     6301 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     6361 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     6421 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     6481 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     6541 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     6601 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     6661 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     6721 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     6781 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagaa
     6841 acggccagcc gaggatatgg aggaagaaca agcctttaaa cgatctcgat aaagcggccg
     6901 cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6961 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     7021 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7081 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7141 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7201 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7261 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     7321 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7381 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7441 gcatcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     7501 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     7561 aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct ttttgcttgt
     7621 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7681 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7741 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7801 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7861 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7921 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7981 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     8041 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8101 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     8161 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     8221 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     8281 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     8341 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     8401 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     8461 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     8521 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     8581 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     8641 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     8701 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     8761 ctattggtta aaaaatgagc tgat
//
