LOCUS       sequence-352757-        8784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.C.S_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    404..637
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             822..866
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1164..1412
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1164..1184
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1335..1354
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        1431..1506
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    1554..1671
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1811..2989
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2042..2980
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2937..2957
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3038..3047
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3086..3121
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             3140..3184
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             3194..3901
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3339..3357)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3607..3626
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3971..4559
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4024..4044)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4442..4453)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4634..4867
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4939..5060
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4976..4995)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5030..5049
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5100..5235
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5255..5274)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5256..5274)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5284..5301)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5284..5300)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5293..5315)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5442..5897
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5529..5548)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5739..5760
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5923..6027
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6028..6096,6097..6888)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6246..6265)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7059..7647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7548..7567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7801..7818
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7935..7956
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7971..7976,7977..7994,7995..8001)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8009..8025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8014..8036
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8033..8049
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8033..8049
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8068..8088
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8070..8088
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8116..8342
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8343..8523
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8570..8695
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
       61 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
      121 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
      181 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
      241 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
      301 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
      361 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
      421 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
      481 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
      541 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
      601 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
      661 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
      721 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
      781 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
      841 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
      901 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
      961 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     1021 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     1081 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagt
     1141 atcgagcggc cgcccccttc accgagggcc tatttcccat gattccttca tatttgcata
     1201 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
     1261 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
     1321 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
     1381 ctttatatat cttgtggaaa ggacgaaaca ccgggtcttc gagaagacct gttttagagc
     1441 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     1501 cggtgctttt tttaattctc gacctcgaga caaatggcag tattcatcca caattttaaa
     1561 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     1621 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     1681 tacagggaca gcagagatcc actttggccg cggctcgagg cgataagctt tgcaaagatg
     1741 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
     1801 agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     1861 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     1921 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     1981 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     2041 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     2101 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
     2161 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
     2221 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
     2281 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
     2341 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
     2401 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     2461 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     2521 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     2581 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     2641 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     2701 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     2761 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     2821 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     2881 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     2941 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattctgca
     3001 gtcgatcgac ggtaccgcgg gcccgggatc cacggtcgcc accatgggcg acacctatcg
     3061 gtacattgga ggaggcgggg gaggggagga ccaggtggac cccaggctga tcgacggcaa
     3121 gggaggaggc gggggaggga aggagaccgc cgccgccaag ttcgagaggc agcacatgga
     3181 cagcggcatg ctgatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     3241 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     3301 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     3361 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     3421 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     3481 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     3541 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     3601 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     3661 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     3721 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     3781 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     3841 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     3901 gaaacggcca gccgaggata tggaggaaga acaagccttt aaacgatctc gataaagcgg
     3961 ccgcgtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4021 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4081 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4141 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4201 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4261 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4321 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     4381 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4441 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4501 tccgcatctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     4561 tgggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     4621 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gataagatct gctttttgct
     4681 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4741 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4801 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4861 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     4921 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4981 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5041 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     5101 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5161 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     5221 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     5281 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5341 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5401 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     5461 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5521 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5581 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5641 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5701 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5761 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     5821 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     5881 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5941 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6001 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6061 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6121 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6181 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6241 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6301 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6361 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6421 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6481 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6541 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6601 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6661 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6721 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6781 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6841 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6901 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6961 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7021 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7081 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7141 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7201 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7261 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7321 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7381 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7441 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7501 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7561 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7621 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7681 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7741 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7801 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7861 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     7921 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     7981 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     8041 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     8101 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     8161 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatggcga ttggtggaag
     8221 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     8281 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     8341 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8401 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8461 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8521 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     8581 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     8641 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     8701 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     8761 aaaatataaa ttaaaacata tagt
//
