LOCUS       sequence-352760-        8763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.HA.HSV_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        61..165
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(166..234,235..1026)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(384..403)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1197..1785
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1686..1705
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1939..1956
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2073..2094
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2109..2114,2115..2132,2133..2139)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2147..2163
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2152..2174
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2171..2187
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2171..2187
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2206..2226
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2208..2226
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2254..2480
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2481..2661
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2708..2833
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3326..3559
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3744..3788
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4086..4334
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4086..4106
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4257..4276
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4353..4428
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4476..4593
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4733..5911
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4964..5902
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5859..5879
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5960..5969
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6008..6034
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6053..6085
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             6095..6802
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6240..6258)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6508..6527
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6872..7460
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6925..6945)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7343..7354)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7535..7768
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7840..7961
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7877..7896)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7931..7950
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8001..8136
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8156..8175)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8157..8175)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8185..8202)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8185..8201)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8194..8216)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8343..8763,1..35)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8430..8449)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8640..8661
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
       61 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      121 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      181 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      241 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      301 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      361 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      421 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      481 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      541 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      601 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      661 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
      721 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
      781 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
      841 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
      901 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      961 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     1021 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1081 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1141 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1201 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1261 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1321 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1381 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1441 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1501 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1561 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1621 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1681 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1741 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1801 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1861 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1921 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     1981 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     2041 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2101 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     2161 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     2221 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     2281 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     2341 catggcgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     2401 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     2461 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     2521 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     2581 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     2641 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     2701 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     2761 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     2821 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     2881 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     2941 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     3001 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     3061 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     3121 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     3181 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     3241 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     3301 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     3361 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     3421 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     3481 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     3541 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     3601 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     3661 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     3721 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     3781 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     3841 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     3901 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     3961 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     4021 gattagtgaa cggatctcga cggtatcgat cacgagacta gtatcgagcg gccgccccct
     4081 tcaccgaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
     4141 gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
     4201 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
     4261 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     4321 aaggacgaaa caccgggtct tcgagaagac ctgttttaga gctagaaata gcaagttaaa
     4381 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttaattc
     4441 tcgacctcga gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     4501 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     4561 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     4621 ccactttggc cgcggctcga ggcgataagc tttgcaaaga tggataaagt tttaaacaga
     4681 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
     4741 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     4801 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     4861 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     4921 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
     4981 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
     5041 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
     5101 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
     5161 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
     5221 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     5281 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     5341 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     5401 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     5461 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     5521 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     5581 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     5641 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     5701 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     5761 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     5821 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     5881 aagttttttt cttccatttc aggtgtcgtg aggaattctg cagtcgatcg acggtaccgc
     5941 gggcccggga tccacggtcg ccaccatggg cgacacctat cggtacattg gaggaggcgg
     6001 gggagggtac ccatacgatg tgccagatta cgctggagga ggcgggggag ggcagcccga
     6061 gctggccccc gaggaccccg aggacggcat gctgatggtg agcaagggcg aggaggataa
     6121 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     6181 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     6241 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     6301 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     6361 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     6421 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     6481 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     6541 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     6601 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     6661 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     6721 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     6781 cggcatggac gagctgtaca agaaacggcc agccgaggat atggaggaag aacaagcctt
     6841 taaacgatct cgataaagcg gccgcgtcga caatcaacct ctggattaca aaatttgtga
     6901 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6961 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     7021 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     7081 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     7141 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     7201 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     7261 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     7321 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     7381 gctgccggct ctgcggcctc ttccgcatct tcgccttcgc cctcagacga gtcggatctc
     7441 cctttgggcc gcctccccgc ctgggtacct ttaagaccaa tgacttacaa ggcagctgta
     7501 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7561 agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     7621 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7681 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7741 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7801 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     7861 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7921 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     7981 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     8041 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     8101 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     8161 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     8221 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     8281 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     8341 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     8401 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     8461 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     8521 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     8581 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     8641 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     8701 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     8761 taa
//
