LOCUS       sequence-352765-        8772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.HA.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        99..203
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(204..272,273..1064)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(422..441)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1235..1823
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1724..1743
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1977..1994
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2111..2132
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2147..2152,2153..2170,2171..2177)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2185..2201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2190..2212
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2209..2225
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2209..2225
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2244..2264
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2246..2264
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2292..2518
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2519..2699
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2746..2871
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3364..3597
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3782..3826
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4124..4372
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4124..4144
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4295..4314
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4391..4466
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4514..4631
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4771..5949
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5002..5940
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5897..5917
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5998..6007
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6046..6072
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6091..6132
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6142..6849
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6287..6305)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6555..6574
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6919..7507
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6972..6992)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7390..7401)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7582..7815
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7887..8008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7924..7943)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7978..7997
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8048..8183
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8203..8222)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8204..8222)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8232..8249)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8232..8248)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8241..8263)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8390..8772,1..73)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8477..8496)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8687..8708
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
       61 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      121 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      181 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      241 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      301 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
      361 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
      421 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
      481 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
      541 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
      601 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
      661 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
      721 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
      781 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
      841 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
      901 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
      961 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1021 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1081 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1141 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1201 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1261 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1321 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1381 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1441 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1501 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1561 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1621 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1681 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1741 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1801 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     1861 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     1921 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     1981 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     2041 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     2101 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     2161 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     2221 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     2281 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     2341 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tggcgattgg tggaagtaag
     2401 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     2461 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     2521 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     2581 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     2641 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     2701 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     2761 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     2821 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     2881 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     2941 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     3001 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     3061 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     3121 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     3181 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     3241 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     3301 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     3361 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     3421 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     3481 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     3541 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     3601 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     3661 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     3721 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     3781 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     3841 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     3901 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     3961 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     4021 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     4081 gtatcgatca cgagactagt atcgagcggc cgcccccttc accgagggcc tatttcccat
     4141 gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt
     4201 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg
     4261 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg
     4321 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgggtcttc
     4381 gagaagacct gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     4441 ttgaaaaagt ggcaccgagt cggtgctttt tttaattctc gacctcgaga caaatggcag
     4501 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     4561 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     4621 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg cggctcgagg
     4681 cgataagctt tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg
     4741 accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc
     4801 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
     4861 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     4921 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     4981 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
     5041 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt
     5101 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
     5161 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
     5221 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
     5281 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
     5341 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
     5401 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
     5461 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc
     5521 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
     5581 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
     5641 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     5701 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     5761 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     5821 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     5881 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     5941 gtgtcgtgag gaattctgca gtcgatcgac ggtaccgcgg gcccgggatc cacggtcgcc
     6001 accatgggcg acacctatcg gtacattgga ggaggcgggg gagggtaccc atacgatgtg
     6061 ccagattacg ctggaggagg cgggggaggg ggcaaaccaa ttccaaaccc actgctggga
     6121 ctggattcca caggcatgct gatggtgagc aagggcgagg aggataacat ggccatcatc
     6181 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     6241 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     6301 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     6361 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     6421 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     6481 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     6541 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     6601 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     6661 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     6721 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     6781 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     6841 ctgtacaaga aacggccagc cgaggatatg gaggaagaac aagcctttaa acgatctcga
     6901 taaagcggcc gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6961 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7021 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7081 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7141 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     7201 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     7261 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     7321 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     7381 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     7441 cggcctcttc cgcatcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7501 tccccgcctg ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7561 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga taagatctgc
     7621 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7681 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7741 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7801 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     7861 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     7921 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7981 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     8041 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8101 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8161 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     8221 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     8281 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     8341 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     8401 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     8461 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8521 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8581 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     8641 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8701 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8761 ccgatttcgg cc
//
