LOCUS       sequence-35277-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 4120 (=HL 716 + pHL 1370)
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    211..227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        285..389
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(608..627)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttctccttt acgcatgcta tcatgaactt tcagcgtatt ctgactcata aggtggctcc
       61 tacccgtgat cccttgacgg aacattcaag caaaagcctg gttccgccga tttatcgctg
      121 cggcaaataa cgcagcgggt ttacggattt ccccttgtaa cgaatttcaa aatgcaagcg
      181 tgttgaactg gttccggtgt cgacagtatc ttgttatccg ctcacaatgt caatgttatc
      241 cgctcacatt tatgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      301 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      361 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      421 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      481 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      541 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
      601 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
      661 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
      721 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
      781 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
      841 acaacatggg ggatcatgta actcgccttg atc
//
