LOCUS       sequence-352771-        8763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.HSV.NWS_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..27
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     24..40
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..40
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     59..79
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        61..79
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        107..333
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             334..514
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    561..686
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1179..1412
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1597..1641
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1939..2187
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1939..1959
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2110..2129
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2206..2281
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2329..2446
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2586..3764
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2817..3755
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3712..3732
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3813..3822
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3861..3893
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             3915..3938
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3948..4655
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4093..4111)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4361..4380
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4725..5313
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4778..4798)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5196..5207)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5388..5621
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5693..5814
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5730..5749)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5784..5803
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5854..5989
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6009..6028)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6010..6028)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6038..6055)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6038..6054)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6047..6069)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6196..6651
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6283..6302)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6493..6514
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6677..6781
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6782..6850,6851..7642)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7000..7019)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7813..8401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8302..8321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8555..8572
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8689..8710
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8725..8730,8731..8748,8749..8755)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    join(8763,1..16)
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
       61 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
      121 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
      181 aaaaagcacc gtgcatggcg attggtggaa gtaaggtggt acgatcgtgc cttattagga
      241 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
      301 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
      361 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      421 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      481 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      541 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      601 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      661 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      721 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      781 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      841 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
      901 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
      961 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1021 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1081 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1141 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1201 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1261 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1321 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1381 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1441 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1501 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1561 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1621 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1681 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1741 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1801 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1861 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagtatcga
     1921 gcggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt gcatatacga
     1981 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
     2041 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
     2101 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
     2161 tatatcttgt ggaaaggacg aaacaccggg tcttcgagaa gacctgtttt agagctagaa
     2221 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
     2281 ctttttttaa ttctcgacct cgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2341 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2401 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2461 ggacagcaga gatccacttt ggccgcggct cgaggcgata agctttgcaa agatggataa
     2521 agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg
     2581 gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
     2641 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
     2701 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     2761 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
     2821 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
     2881 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
     2941 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
     3001 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
     3061 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
     3121 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
     3181 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
     3241 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
     3301 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
     3361 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
     3421 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
     3481 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
     3541 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     3601 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     3661 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     3721 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaggaatt ctgcagtcga
     3781 tcgacggtac cgcgggcccg ggatccacgg tcgccaccat gggcgacacc tatcggtaca
     3841 ttggaggagg cgggggaggg cagcccgagc tggcccccga ggaccccgag gacggaggag
     3901 gcgggggagg gaactggtcc cacccccaat tcgagaaagg catgctgatg gtgagcaagg
     3961 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     4021 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     4081 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     4141 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     4201 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     4261 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     4321 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     4381 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     4441 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     4501 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     4561 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     4621 gccactccac cggcggcatg gacgagctgt acaagaaacg gccagccgag gatatggagg
     4681 aagaacaagc ctttaaacga tctcgataaa gcggccgcgt cgacaatcaa cctctggatt
     4741 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     4801 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4861 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     4921 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     4981 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     5041 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     5101 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     5161 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     5221 cttcccgcgg cctgctgccg gctctgcggc ctcttccgca tcttcgcctt cgccctcaga
     5281 cgagtcggat ctccctttgg gccgcctccc cgcctgggta cctttaagac caatgactta
     5341 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5401 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
     5461 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5521 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     5581 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     5641 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     5701 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     5761 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     5821 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5881 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5941 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     6001 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     6061 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     6121 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     6181 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6241 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6301 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6361 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6421 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6481 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6541 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6601 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     6661 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6721 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6781 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6841 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6901 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6961 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7021 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7081 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7141 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7201 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7261 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7321 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7381 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7441 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7501 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7561 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7621 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7681 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7741 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7801 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7861 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7921 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7981 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8041 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8101 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8161 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8221 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8281 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8341 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8401 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8461 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8521 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8581 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8641 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8701 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8761 aat
//
