LOCUS       sequence-352772-        8781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.HSV.Ollas_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        100..204
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(205..273,274..1065)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(423..442)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1236..1824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1725..1744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1978..1995
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2112..2133
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2148..2153,2154..2171,2172..2178)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2186..2202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2191..2213
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2210..2226
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2210..2226
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2245..2265
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2247..2265
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2293..2519
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2520..2700
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2747..2872
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3365..3598
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3783..3827
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4125..4373
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4125..4145
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4296..4315
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4392..4467
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4515..4632
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4772..5950
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5003..5941
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5898..5918
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5999..6008
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6047..6079
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             6098..6139
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             6152..6859
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6297..6315)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6565..6584
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6929..7517
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6982..7002)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7400..7411)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7592..7825
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7897..8018
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7934..7953)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7988..8007
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8058..8193
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8213..8232)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8214..8232)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8242..8259)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8242..8258)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8251..8273)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8400..8781,1..74)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8487..8506)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8697..8718
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
       61 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      121 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      181 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      241 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      301 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      361 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      421 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
      481 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      541 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      601 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      661 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
      721 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
      781 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
      841 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
      901 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
      961 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     1021 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     1081 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1141 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1201 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1261 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1321 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1381 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1441 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1501 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1561 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1621 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1681 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1741 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1801 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1861 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1921 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1981 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     2041 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     2101 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     2161 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     2221 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     2281 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     2341 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atggcgattg gtggaagtaa
     2401 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     2461 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     2521 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     2581 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     2641 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     2701 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     2761 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     2821 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     2881 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     2941 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     3001 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     3061 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     3121 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     3181 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     3241 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     3301 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     3361 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     3421 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     3481 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     3541 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     3601 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     3661 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     3721 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     3781 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     3841 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     3901 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     3961 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     4021 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     4081 ggtatcgatc acgagactag tatcgagcgg ccgccccctt caccgagggc ctatttccca
     4141 tgattccttc atatttgcat atacgataca aggctgttag agagataatt ggaattaatt
     4201 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
     4261 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
     4321 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accgggtctt
     4381 cgagaagacc tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     4441 cttgaaaaag tggcaccgag tcggtgcttt ttttaattct cgacctcgag acaaatggca
     4501 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     4561 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     4621 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag
     4681 gcgataagct ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg
     4741 gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg
     4801 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta
     4861 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     4921 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     4981 cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt
     5041 tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct
     5101 tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc
     5161 cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat
     5221 ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt
     5281 ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga
     5341 tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca
     5401 gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta
     5461 gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc
     5521 ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc
     5581 cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga
     5641 gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac
     5701 ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc
     5761 tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac
     5821 tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt
     5881 tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca
     5941 ggtgtcgtga ggaattctgc agtcgatcga cggtaccgcg ggcccgggat ccacggtcgc
     6001 caccatgggc gacacctatc ggtacattgg aggaggcggg ggagggcagc ccgagctggc
     6061 ccccgaggac cccgaggacg gaggaggcgg gggagggagc ggcttcgcca acgagctggg
     6121 ccccaggctg atgggcaagt gcggcatgct gatggtgagc aagggcgagg aggataacat
     6181 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     6241 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     6301 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     6361 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     6421 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     6481 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     6541 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     6601 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     6661 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     6721 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     6781 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     6841 catggacgag ctgtacaaga aacggccagc cgaggatatg gaggaagaac aagcctttaa
     6901 acgatctcga taaagcggcc gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     6961 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     7021 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     7081 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     7141 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     7201 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     7261 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     7321 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     7381 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     7441 gccggctctg cggcctcttc cgcatcttcg ccttcgccct cagacgagtc ggatctccct
     7501 ttgggccgcc tccccgcctg ggtaccttta agaccaatga cttacaaggc agctgtagat
     7561 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     7621 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     7681 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7741 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7801 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     7861 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     7921 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7981 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     8041 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     8101 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     8161 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     8221 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     8281 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     8341 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     8401 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     8461 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     8521 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     8581 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8641 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     8701 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     8761 agggattttg ccgatttcgg c
//
