LOCUS       sequence-352775-        8769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.HSV.VSVg_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(56..75)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(57..75)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(85..102)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(85..101)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(94..116)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      243..698
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(330..349)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     540..561
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        724..828
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(829..897,898..1689)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1047..1066)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1860..2448
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2349..2368
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2602..2619
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2736..2757
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2772..2777,2778..2795,2796..2802)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2810..2826
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2815..2837
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2834..2850
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2834..2850
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2869..2889
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2871..2889
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2917..3143
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3144..3324
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3371..3496
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3989..4222
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4407..4451
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4749..4997
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4749..4769
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4920..4939
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5016..5091
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    5139..5256
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5396..6574
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5627..6565
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6522..6542
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6623..6632
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6671..6703
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             6722..6754
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             6764..7471
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6909..6927)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7177..7196
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7541..8129
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7594..7614)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8012..8023)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8204..8437
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8509..8630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8546..8565)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8600..8619
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      join(8670..8769,1..36)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
       61 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
      121 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
      181 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
      241 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      301 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      361 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      421 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      481 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      541 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      601 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
      661 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
      721 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      781 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      841 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      901 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      961 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     1021 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     1081 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     1141 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     1201 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1261 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1321 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1381 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1441 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1501 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1561 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1621 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1681 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1741 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1801 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1861 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1921 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1981 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2041 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2101 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2161 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2221 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2281 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2341 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2401 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2461 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2521 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2581 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     2641 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     2701 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     2761 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     2821 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     2881 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     2941 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     3001 gtgcatggcg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     3061 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     3121 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     3181 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     3241 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     3301 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     3361 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     3421 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     3481 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     3541 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     3601 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     3661 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     3721 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     3781 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     3841 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     3901 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     3961 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     4021 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     4081 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     4141 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     4201 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     4261 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     4321 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     4381 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     4441 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     4501 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     4561 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     4621 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     4681 ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagtatcga gcggccgccc
     4741 ccttcaccga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
     4801 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     4861 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     4921 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     4981 ggaaaggacg aaacaccggg tcttcgagaa gacctgtttt agagctagaa atagcaagtt
     5041 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttaa
     5101 ttctcgacct cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     5161 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     5221 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     5281 gatccacttt ggccgcggct cgaggcgata agctttgcaa agatggataa agttttaaac
     5341 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc
     5401 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     5461 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     5521 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     5581 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg
     5641 tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc
     5701 cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag
     5761 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg
     5821 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
     5881 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
     5941 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg
     6001 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
     6061 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
     6121 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
     6181 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
     6241 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
     6301 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     6361 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
     6421 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     6481 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     6541 tcaaagtttt tttcttccat ttcaggtgtc gtgaggaatt ctgcagtcga tcgacggtac
     6601 cgcgggcccg ggatccacgg tcgccaccat gggcgacacc tatcggtaca ttggaggagg
     6661 cgggggaggg cagcccgagc tggcccccga ggaccccgag gacggaggag gcgggggagg
     6721 gtatacagat atcgaaatga atagactcgg aaagggcatg ctgatggtga gcaagggcga
     6781 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     6841 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     6901 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     6961 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     7021 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     7081 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     7141 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     7201 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     7261 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     7321 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     7381 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     7441 ctccaccggc ggcatggacg agctgtacaa gaaacggcca gccgaggata tggaggaaga
     7501 acaagccttt aaacgatctc gataaagcgg ccgcgtcgac aatcaacctc tggattacaa
     7561 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     7621 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7681 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7741 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7801 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7861 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7921 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     7981 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     8041 ccgcggcctg ctgccggctc tgcggcctct tccgcatctt cgccttcgcc ctcagacgag
     8101 tcggatctcc ctttgggccg cctccccgcc tgggtacctt taagaccaat gacttacaag
     8161 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     8221 tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     8281 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     8341 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8401 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     8461 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     8521 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8581 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     8641 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     8701 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     8761 cctctgagc
//
