LOCUS       sequence-352776-        8778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.HSV.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     157..176
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    521..1109
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(574..594)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(992..1003)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1184..1417
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1489..1610
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1526..1545)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1580..1599
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1650..1785
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1805..1824)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1806..1824)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1834..1851)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1834..1850)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1843..1865)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1992..2447
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2079..2098)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2289..2310
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2473..2577
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2578..2646,2647..3438)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2796..2815)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3609..4197
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4098..4117
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4351..4368
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4485..4506
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4521..4526,4527..4544,4545..4551)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4559..4575
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4564..4586
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4583..4599
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4583..4599
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4618..4638
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4620..4638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4666..4892
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4893..5073
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5120..5245
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5738..5971
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6156..6200
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6498..6746
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6498..6518
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6669..6688
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        6765..6840
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    6888..7005
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7145..8323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7376..8314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8271..8291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      8372..8381
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8420..8452
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             8471..8512
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             join(8522..8778,1..451)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8667..8685)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
       61 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
      121 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
      181 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
      241 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
      301 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
      361 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
      421 ctccaccggc ggcatggacg agctgtacaa gaaacggcca gccgaggata tggaggaaga
      481 acaagccttt aaacgatctc gataaagcgg ccgcgtcgac aatcaacctc tggattacaa
      541 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
      601 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
      661 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
      721 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
      781 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
      841 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
      901 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
      961 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     1021 ccgcggcctg ctgccggctc tgcggcctct tccgcatctt cgccttcgcc ctcagacgag
     1081 tcggatctcc ctttgggccg cctccccgcc tgggtacctt taagaccaat gacttacaag
     1141 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     1201 tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     1261 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     1321 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     1381 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     1441 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     1501 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     1561 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     1621 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     1681 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     1741 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     1801 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     1861 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     1921 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     1981 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2041 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     2101 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2161 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     2221 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2281 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     2341 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     2401 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     2461 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2521 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2581 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2641 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2701 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2761 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2821 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2881 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2941 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3001 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3061 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3121 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3181 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3241 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3301 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3361 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3421 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3481 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3541 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3601 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3661 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3721 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3781 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3841 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3901 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3961 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4021 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4081 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4141 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4201 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4261 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4321 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4381 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     4441 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     4501 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     4561 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     4621 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
     4681 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
     4741 aaaagcaccg tgcatggcga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
     4801 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
     4861 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
     4921 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     4981 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5041 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     5101 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     5161 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     5221 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     5281 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     5341 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     5401 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     5461 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     5521 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     5581 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     5641 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     5701 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     5761 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     5821 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     5881 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     5941 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     6001 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     6061 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     6121 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     6181 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     6241 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     6301 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     6361 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     6421 acagatccat tcgattagtg aacggatctc gacggtatcg atcacgagac tagtatcgag
     6481 cggccgcccc cttcaccgag ggcctatttc ccatgattcc ttcatatttg catatacgat
     6541 acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag atattagtac
     6601 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
     6661 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
     6721 atatcttgtg gaaaggacga aacaccgggt cttcgagaag acctgtttta gagctagaaa
     6781 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     6841 tttttttaat tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa
     6901 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     6961 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     7021 gacagcagag atccactttg gccgcggctc gaggcgataa gctttgcaaa gatggataaa
     7081 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     7141 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     7201 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     7261 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     7321 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     7381 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     7441 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     7501 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     7561 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     7621 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     7681 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     7741 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     7801 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     7861 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     7921 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     7981 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     8041 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     8101 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     8161 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     8221 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     8281 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggaattc tgcagtcgat
     8341 cgacggtacc gcgggcccgg gatccacggt cgccaccatg ggcgacacct atcggtacat
     8401 tggaggaggc gggggagggc agcccgagct ggcccccgag gaccccgagg acggaggagg
     8461 cgggggaggg ggcaaaccaa ttccaaaccc actgctggga ctggattcca caggcatgct
     8521 gatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     8581 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     8641 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     8701 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     8761 ccccgccgac atccccga
//
