LOCUS       sequence-352777-        8775 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.NWS.Ollas_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        104..208
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(209..277,278..1069)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(427..446)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1240..1828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1729..1748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1982..1999
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2116..2137
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2152..2157,2158..2175,2176..2182)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2190..2206
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2195..2217
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2214..2230
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2214..2230
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2249..2269
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2251..2269
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2297..2523
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2524..2704
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2751..2876
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3369..3602
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3787..3831
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4129..4377
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4129..4149
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4300..4319
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4396..4471
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4519..4636
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4776..5954
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5007..5945
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5902..5922
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6003..6012
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6054..6077
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6096..6137
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             6150..6857
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6295..6313)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6563..6582
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6927..7515
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6980..7000)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7398..7409)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7590..7823
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7895..8016
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7932..7951)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7986..8005
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8056..8191
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8211..8230)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8212..8230)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8240..8257)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8240..8256)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8249..8271)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8398..8775,1..78)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8485..8504)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8695..8716
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
       61 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     2041 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     2101 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     2161 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     2221 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     2281 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     2341 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatggcg attggtggaa
     2401 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     2461 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
     2521 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     2581 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     2641 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     2701 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     2761 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     2821 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     2881 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     2941 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     3001 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     3061 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     3121 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     3181 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     3241 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     3301 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     3361 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     3421 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     3481 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     3541 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     3601 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     3661 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     3721 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     3781 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     3841 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     3901 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     3961 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     4021 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     4081 cgacggtatc gatcacgaga ctagtatcga gcggccgccc ccttcaccga gggcctattt
     4141 cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt
     4201 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt
     4261 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta
     4321 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccggg
     4381 tcttcgagaa gacctgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
     4441 tcaacttgaa aaagtggcac cgagtcggtg ctttttttaa ttctcgacct cgagacaaat
     4501 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     4561 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     4621 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggccgcggct
     4681 cgaggcgata agctttgcaa agatggataa agttttaaac agagaggaat ctttgcagct
     4741 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
     4801 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     4861 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     4921 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     4981 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     5041 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     5101 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     5161 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     5221 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     5281 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     5341 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     5401 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     5461 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     5521 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     5581 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     5641 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     5701 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     5761 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     5821 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     5881 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     5941 ttcaggtgtc gtgaggaatt ctgcagtcga tcgacggtac cgcgggcccg ggatccacgg
     6001 tcgccaccat gggcgacacc tatcggtaca ttggaggagg cgggggaggg aactggtccc
     6061 acccccaatt cgagaaagga ggaggcgggg gagggagcgg cttcgccaac gagctgggcc
     6121 ccaggctgat gggcaagtgc ggcatgctga tggtgagcaa gggcgaggag gataacatgg
     6181 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     6241 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     6301 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     6361 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     6421 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     6481 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     6541 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     6601 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     6661 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     6721 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     6781 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     6841 tggacgagct gtacaagaaa cggccagccg aggatatgga ggaagaacaa gcctttaaac
     6901 gatctcgata aagcggccgc gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     6961 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     7021 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     7081 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     7141 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     7201 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     7261 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     7321 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     7381 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7441 cggctctgcg gcctcttccg catcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7501 gggccgcctc cccgcctggg tacctttaag accaatgact tacaaggcag ctgtagatct
     7561 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata
     7621 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7681 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7741 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7801 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     7861 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     7921 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7981 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     8041 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8101 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     8161 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     8221 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     8281 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     8341 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     8401 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     8461 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     8521 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     8581 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     8641 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     8701 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     8761 ggattttgcc gattt
//
