LOCUS       sequence-352779-        8763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.NWS.VSVg_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        43..147
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(148..216,217..1008)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(366..385)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1179..1767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1668..1687
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1921..1938
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2055..2076
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2091..2096,2097..2114,2115..2121)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2129..2145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2156
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2153..2169
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2153..2169
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2188..2208
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2190..2208
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2236..2462
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2463..2643
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2690..2815
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3308..3541
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3726..3770
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4068..4316
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4068..4088
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4239..4258
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4335..4410
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4458..4575
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4715..5893
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4946..5884
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5841..5861
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5942..5951
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5993..6016
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6035..6067
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             6077..6784
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6222..6240)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6490..6509
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6854..7442
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6907..6927)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7325..7336)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7517..7750
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7822..7943
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7859..7878)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7913..7932
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7983..8118
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8138..8157)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8139..8157)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8167..8184)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8167..8183)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8176..8198)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8325..8753
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8412..8431)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8622..8643
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
       61 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      121 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      181 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      241 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
      301 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
      361 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
      421 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
      481 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
      541 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
      601 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
      661 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
      721 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
      781 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
      841 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
      901 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
      961 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     1021 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     1081 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1141 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1201 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1261 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1321 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1381 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1441 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1501 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1561 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1621 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1681 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1741 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1801 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     1861 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     1921 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     1981 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     2041 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     2101 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     2161 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     2221 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     2281 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatggcga ttggtggaag
     2341 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     2401 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     2461 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     2521 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     2581 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     2641 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     2701 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     2761 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     2821 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     2881 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     2941 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     3001 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     3061 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     3121 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     3181 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     3241 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     3301 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     3361 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     3421 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     3481 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     3541 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     3601 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     3661 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     3721 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     3781 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     3841 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     3901 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     3961 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
     4021 gacggtatcg atcacgagac tagtatcgag cggccgcccc cttcaccgag ggcctatttc
     4081 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attggaatta
     4141 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc
     4201 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa
     4261 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgggt
     4321 cttcgagaag acctgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     4381 caacttgaaa aagtggcacc gagtcggtgc tttttttaat tctcgacctc gagacaaatg
     4441 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     4501 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     4561 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc
     4621 gaggcgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
     4681 atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc agtgggcaga
     4741 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
     4801 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     4861 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     4921 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
     4981 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
     5041 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
     5101 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
     5161 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
     5221 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
     5281 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
     5341 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
     5401 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
     5461 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     5521 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     5581 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     5641 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     5701 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     5761 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     5821 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     5881 tcaggtgtcg tgaggaattc tgcagtcgat cgacggtacc gcgggcccgg gatccacggt
     5941 cgccaccatg ggcgacacct atcggtacat tggaggaggc gggggaggga actggtccca
     6001 cccccaattc gagaaaggag gaggcggggg agggtataca gatatcgaaa tgaatagact
     6061 cggaaagggc atgctgatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     6121 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     6181 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     6241 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     6301 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     6361 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     6421 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     6481 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     6541 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     6601 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     6661 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     6721 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     6781 caagaaacgg ccagccgagg atatggagga agaacaagcc tttaaacgat ctcgataaag
     6841 cggccgcgtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6901 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6961 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     7021 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     7081 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7141 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7201 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     7261 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7321 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7381 tcttccgcat cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7441 gcctgggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7501 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga tctgcttttt
     7561 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7621 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7681 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7741 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7801 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     7861 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7921 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     7981 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8041 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     8101 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     8161 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     8221 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     8281 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     8341 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     8401 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     8461 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     8521 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     8581 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     8641 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     8701 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     8761 aat
//
