LOCUS       sequence-35278-e         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3904 (=HL 770 + pHL 991)
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    252..270
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(291..309)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        377..481
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(700..719)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttctccttt acgcatgctc gcggtacctt taatcaggtt gttcaggtta gtgacataag
       61 tctgatggtg cttgccgtag tgatactcga tggtttccgc agaaatgtgc ggtgccagag
      121 catctttagc atatggtagt gcaggtaatt cgaatgacat tgctactctc ctttattatt
      181 aatttgcata cgtacaatag ccttattgtg cgtatggtcg acagtctctc tatcactgat
      241 agggatgtca atctctatca ctgataggga ctcgaggtga agacgaaagg gcctcgtgat
      301 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      361 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      421 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      481 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      541 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      601 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      661 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      721 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      781 ggttgagtac tca
//
