LOCUS       sequence-352780-        8772 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.NWS.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        103..207
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(208..276,277..1068)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(426..445)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1239..1827
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1728..1747
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1981..1998
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2115..2136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2151..2156,2157..2174,2175..2181)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2189..2205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2194..2216
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2213..2229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2213..2229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2248..2268
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2250..2268
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2296..2522
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2523..2703
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2750..2875
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3368..3601
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3786..3830
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4128..4376
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4128..4148
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4299..4318
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4395..4470
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4518..4635
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4775..5953
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5006..5944
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5901..5921
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6002..6011
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6053..6076
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6095..6136
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6146..6853
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6291..6309)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6559..6578
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6923..7511
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6976..6996)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7394..7405)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7586..7819
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7891..8012
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7928..7947)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7982..8001
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8052..8187
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8207..8226)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8208..8226)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8236..8253)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8236..8252)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8245..8267)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8394..8772,1..77)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8481..8500)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8691..8712
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
       61 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      121 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      181 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      241 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      301 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
      361 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
      421 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
      481 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
      541 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
      601 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
      661 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
      721 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
      781 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
      841 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
      901 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
      961 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     1021 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     1081 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     1141 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1201 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1261 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1321 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1381 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1441 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1501 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1561 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1621 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1681 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1741 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1801 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1861 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     1921 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     1981 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     2041 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     2101 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     2161 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     2221 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     2281 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     2341 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatggcga ttggtggaag
     2401 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     2461 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     2521 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     2581 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     2641 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     2701 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     2761 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     2821 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     2881 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     2941 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     3001 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     3061 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     3121 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     3181 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     3241 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     3301 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     3361 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     3421 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     3481 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     3541 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     3601 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     3661 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     3721 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     3781 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     3841 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     3901 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     3961 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     4021 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
     4081 gacggtatcg atcacgagac tagtatcgag cggccgcccc cttcaccgag ggcctatttc
     4141 ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata attggaatta
     4201 atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc
     4261 ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa
     4321 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga aacaccgggt
     4381 cttcgagaag acctgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     4441 caacttgaaa aagtggcacc gagtcggtgc tttttttaat tctcgacctc gagacaaatg
     4501 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     4561 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     4621 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc
     4681 gaggcgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
     4741 atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc agtgggcaga
     4801 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
     4861 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     4921 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     4981 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
     5041 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
     5101 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
     5161 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
     5221 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
     5281 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
     5341 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
     5401 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
     5461 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
     5521 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     5581 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     5641 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     5701 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     5761 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     5821 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     5881 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     5941 tcaggtgtcg tgaggaattc tgcagtcgat cgacggtacc gcgggcccgg gatccacggt
     6001 cgccaccatg ggcgacacct atcggtacat tggaggaggc gggggaggga actggtccca
     6061 cccccaattc gagaaaggag gaggcggggg agggggcaaa ccaattccaa acccactgct
     6121 gggactggat tccacaggca tgctgatggt gagcaagggc gaggaggata acatggccat
     6181 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     6241 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     6301 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     6361 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     6421 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     6481 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     6541 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     6601 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     6661 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     6721 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     6781 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     6841 cgagctgtac aagaaacggc cagccgagga tatggaggaa gaacaagcct ttaaacgatc
     6901 tcgataaagc ggccgcgtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     6961 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     7021 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     7081 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     7141 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7201 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7261 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     7321 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7381 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7441 tctgcggcct cttccgcatc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7501 cgcctccccg cctgggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     7561 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagataagat
     7621 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7681 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7741 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7801 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     7861 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     7921 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7981 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     8041 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     8101 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     8161 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     8221 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     8281 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     8341 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     8401 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     8461 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     8521 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8581 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8641 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8701 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8761 tttgccgatt tc
//
