LOCUS       sequence-352782-        8793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.Ollas.S_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..236
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(237..305,306..1097)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(455..474)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1268..1856
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1757..1776
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2010..2027
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2144..2165
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2180..2185,2186..2203,2204..2210)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2218..2234
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2223..2245
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2242..2258
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2242..2258
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2277..2297
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2279..2297
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2325..2551
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2552..2732
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2779..2904
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3397..3630
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3815..3859
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4157..4405
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4157..4177
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4328..4347
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4424..4499
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4547..4664
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4804..5982
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5035..5973
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5930..5950
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6031..6040
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6079..6120
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             6142..6186
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             6196..6903
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6341..6359)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6609..6628
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6973..7561
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7026..7046)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7444..7455)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7636..7869
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7941..8062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7978..7997)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8032..8051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8102..8237
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8257..8276)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8258..8276)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8286..8303)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8286..8302)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8295..8317)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8444..8793,1..106)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8531..8550)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8741..8762
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
       61 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
      121 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      181 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
      241 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      301 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      361 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      421 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
      481 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      541 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
      601 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
      661 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
      721 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
      781 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
      841 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
      901 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
      961 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1021 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1081 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1141 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1201 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1261 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1321 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1381 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1441 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1501 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1561 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1621 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1681 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1741 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1801 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1861 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1921 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1981 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     2041 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     2101 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     2161 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     2221 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     2281 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     2341 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     2401 aaagcaccgt gcatggcgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     2461 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     2521 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     2581 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     2641 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     2701 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     2761 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     2821 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     2881 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     2941 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     3001 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     3061 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     3121 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     3181 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     3241 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     3301 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     3361 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     3421 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     3481 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     3541 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     3601 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     3661 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     3721 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     3781 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     3841 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     3901 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     3961 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     4021 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     4081 cagatccatt cgattagtga acggatctcg acggtatcga tcacgagact agtatcgagc
     4141 ggccgccccc ttcaccgagg gcctatttcc catgattcct tcatatttgc atatacgata
     4201 caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca
     4261 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
     4321 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
     4381 tatcttgtgg aaaggacgaa acaccgggtc ttcgagaaga cctgttttag agctagaaat
     4441 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     4501 ttttttaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     4561 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     4621 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     4681 acagcagaga tccactttgg ccgcggctcg aggcgataag ctttgcaaag atggataaag
     4741 ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga
     4801 attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     4861 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     4921 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     4981 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
     5041 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
     5101 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
     5161 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
     5221 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
     5281 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
     5341 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
     5401 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
     5461 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
     5521 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
     5581 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     5641 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     5701 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     5761 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     5821 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     5881 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     5941 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggaattct gcagtcgatc
     6001 gacggtaccg cgggcccggg atccacggtc gccaccatgg gcgacaccta tcggtacatt
     6061 ggaggaggcg ggggagggag cggcttcgcc aacgagctgg gccccaggct gatgggcaag
     6121 tgcggaggag gcgggggagg gaaggagacc gccgccgcca agttcgagag gcagcacatg
     6181 gacagcggca tgctgatggt gagcaagggc gaggaggata acatggccat catcaaggag
     6241 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     6301 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     6361 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     6421 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     6481 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     6541 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     6601 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     6661 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     6721 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     6781 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     6841 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     6901 aagaaacggc cagccgagga tatggaggaa gaacaagcct ttaaacgatc tcgataaagc
     6961 ggccgcgtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     7021 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     7081 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     7141 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7201 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7261 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7321 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     7381 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7441 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7501 cttccgcatc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7561 cctgggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7621 aagaaaaggg gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg
     7681 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7741 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7801 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7861 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     7921 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     7981 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8041 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     8101 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     8161 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     8221 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     8281 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     8341 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     8401 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     8461 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     8521 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     8581 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     8641 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     8701 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     8761 ggaacaacac tcaaccctat ctcggtctat tct
//
