LOCUS       sequence-352786-        4825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector backbone. pClgn-promoter driven expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Zhang X, Sun J, Lu Y, Zhang J, Shimada K, Noda T, Zhao S, Koyano T, 
            Matsuyama M, Zhou S, Wu J, Ikawa M, Liu M
  TITLE     LRRC23 is a conserved component of the radial spoke that is 
            necessary for sperm motility and male fertility in mice.
  JOURNAL   J Cell Sci. 2021 Oct 15;134(20). pii: 272563. doi: 
            10.1242/jcs.259381. Epub 2021 Oct 21.
  PUBMED    34585727
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-15"; volume: "134(20). pii: 272563. doi"; 
            pages: " 10.1242/jcs.259381. Epub 2021 Oct 21"
COMMENT     Sequence Label: pClgn-Lrrc23-1D4
FEATURES             Location/Qualifiers
     source          1..4825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1505..1524)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1570..1625
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1624..1643)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1980..1999)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1981..1999)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2009..2026)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2009..2025)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2018..2040)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2167..2622
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2254..2273)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2464..2485
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2648..2752
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2753..2821,2822..3613)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2971..2990)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3784..4372
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4273..4292
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4526..4543
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4660..4681
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4696..4701,4702..4719,4720..4726)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4734..4750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4739..4761
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4758..4774
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4758..4774
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4793..4813
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4795..4813
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagctcatcg atgagctcca gagagggact gacgttggaa ggaccgcatc cccccaggcg
       61 cgcggccgct gggtcccgag cggcggttga gcgggccgct tgcgcactgg ggctgccact
      121 gcatcctgca tcctccggcg cggccgctcg cgcacgcgca gttagcagtc gctgctgggc
      181 ggctgccggc gggatcgctc tggggagacg ccgggcacca agtagcggcc agtcggacac
      241 ctgagctgcc gctgctggac acaagcccgc aggacaggtg tgtagaccgc gggccgcctt
      301 cctgcggctt gttctctggg gaccgggtga gaaatcggat ctctagagcc gccatgtcgg
      361 atgaagatga tgtggatgac gttgatgcag aacaggatga agtggaaagt gacaaggaga
      421 tagaggaatg ggaagactac aggaaagaga cagaagaagc ctctgaggaa tggctgccca
      481 cccccatcac ggaggccatg atgaaggaag ggctctcatt actctgcaag attggcagtg
      541 gactggctca cgcttatatt aagctggagg ctaaggacag ggacctcaca gacatctcct
      601 tactgcgctc ctatatccat cttcgctatg tggatatttc tgagaaccat attacagaca
      661 tatcgccgct caacagtctc actcacctcc tatggctcaa ggccgatggc aatcagcttc
      721 ggagtgctag gatgaatgaa cttccctacc tgcagatcgc cagcttttcc tacaaccaga
      781 tcatagacac tgaaggcatc tttcaccccc gtctgggaag cctggatctc aaagggaacc
      841 gcatccacca ggtgactggt ctggaccccg agagactgtc gagcctgcac accctggagc
      901 ttcgagggaa ccagctggaa tccacaaagg gaatctacct tcctaagctg aagaatctct
      961 acctggctca aaacctgctg aagaaggtag agggcctgga gaacctgagc aacttgacca
     1021 ccctgcatct gcgagacaac cagattgaaa ctttgaatgg cttctcccag gaaatgaagt
     1081 cgctgcagta cctcaacttg aggagcaata tgattagtga tctggcagag ctggccaaac
     1141 tgcgggacct gcccaaactt cgagccctgg tgctgctgga caacccatgt gcggatgaga
     1201 ccgactaccg ccaggaggcc ctggtgcaga tggcgcacct tgagcgcctg gacaaagaat
     1261 tctatgagga tgatgaccgg gcagaggccg aggagatccg tcagaggctg aaggaggaac
     1321 aggatcaaga tctcgatccg gaccaggaca tggaaccgta cttgccacca gttgatatcg
     1381 ggggcggggg cacagagacc agccaagtgg cgcctgccta gctcgagact cctcaggtgc
     1441 aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact
     1501 gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga
     1561 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
     1621 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg
     1681 tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa
     1741 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct
     1801 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc
     1861 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag
     1921 tcatagctgt ccctcttctc ttatgaagat ccctcgactt aattaaggta cccaattcgc
     1981 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     2041 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     2101 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     2161 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     2221 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     2281 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     2341 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     2401 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     2461 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     2521 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     2581 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     2641 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     2701 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     2761 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2821 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2881 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2941 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     3001 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     3061 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3121 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3181 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3241 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3301 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3361 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3421 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3481 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3541 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3601 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3661 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3721 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3781 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3841 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3901 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3961 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4021 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4081 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4141 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4201 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4261 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4321 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4381 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4441 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4501 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     4561 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     4621 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     4681 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     4741 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     4801 ccctcactaa agggaacaaa agctg
//
