LOCUS       sequence-352788-        4810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector backbone. pClgn-promoter driven expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Zhang X, Sun J, Lu Y, Zhang J, Shimada K, Noda T, Zhao S, Koyano T, 
            Matsuyama M, Zhou S, Wu J, Ikawa M, Liu M
  TITLE     LRRC23 is a conserved component of the radial spoke that is 
            necessary for sperm motility and male fertility in mice.
  JOURNAL   J Cell Sci. 2021 Oct 15;134(20). pii: 272563. doi: 
            10.1242/jcs.259381. Epub 2021 Oct 21.
  PUBMED    34585727
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-15"; volume: "134(20). pii: 272563. doi"; 
            pages: " 10.1242/jcs.259381. Epub 2021 Oct 21"
COMMENT     Sequence Label: pClgn-Lrrc23-FLAG
FEATURES             Location/Qualifiers
     source          1..4810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1380..1403
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(1490..1509)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1555..1610
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1609..1628)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1965..1984)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1966..1984)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1994..2011)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1994..2010)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2003..2025)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2152..2607
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2239..2258)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2449..2470
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2633..2737
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2738..2806,2807..3598)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2956..2975)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3769..4357
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4258..4277
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4511..4528
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4645..4666
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4681..4686,4687..4704,4705..4711)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4719..4735
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4724..4746
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4743..4759
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4743..4759
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4778..4798
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4780..4798
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gagctcatcg atgagctcca gagagggact gacgttggaa ggaccgcatc cccccaggcg
       61 cgcggccgct gggtcccgag cggcggttga gcgggccgct tgcgcactgg ggctgccact
      121 gcatcctgca tcctccggcg cggccgctcg cgcacgcgca gttagcagtc gctgctgggc
      181 ggctgccggc gggatcgctc tggggagacg ccgggcacca agtagcggcc agtcggacac
      241 ctgagctgcc gctgctggac acaagcccgc aggacaggtg tgtagaccgc gggccgcctt
      301 cctgcggctt gttctctggg gaccgggtga gaaatcggat ctctagagcc gccatgtcgg
      361 atgaagatga tgtggatgac gttgatgcag aacaggatga agtggaaagt gacaaggaga
      421 tagaggaatg ggaagactac aggaaagaga cagaagaagc ctctgaggaa tggctgccca
      481 cccccatcac ggaggccatg atgaaggaag ggctctcatt actctgcaag attggcagtg
      541 gactggctca cgcttatatt aagctggagg ctaaggacag ggacctcaca gacatctcct
      601 tactgcgctc ctatatccat cttcgctatg tggatatttc tgagaaccat attacagaca
      661 tatcgccgct caacagtctc actcacctcc tatggctcaa ggccgatggc aatcagcttc
      721 ggagtgctag gatgaatgaa cttccctacc tgcagatcgc cagcttttcc tacaaccaga
      781 tcatagacac tgaaggcatc tttcaccccc gtctgggaag cctggatctc aaagggaacc
      841 gcatccacca ggtgactggt ctggaccccg agagactgtc gagcctgcac accctggagc
      901 ttcgagggaa ccagctggaa tccacaaagg gaatctacct tcctaagctg aagaatctct
      961 acctggctca aaacctgctg aagaaggtag agggcctgga gaacctgagc aacttgacca
     1021 ccctgcatct gcgagacaac cagattgaaa ctttgaatgg cttctcccag gaaatgaagt
     1081 cgctgcagta cctcaacttg aggagcaata tgattagtga tctggcagag ctggccaaac
     1141 tgcgggacct gcccaaactt cgagccctgg tgctgctgga caacccatgt gcggatgaga
     1201 ccgactaccg ccaggaggcc ctggtgcaga tggcgcacct tgagcgcctg gacaaagaat
     1261 tctatgagga tgatgaccgg gcagaggccg aggagatccg tcagaggctg aaggaggaac
     1321 aggatcaaga tctcgatccg gaccaggaca tggaaccgta cttgccacca gttgatatcg
     1381 actacaagga tgacgatgac aagtagctcg agactcctca ggtgcaggct gcctatcaga
     1441 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct
     1501 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     1561 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     1621 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     1681 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     1741 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     1801 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     1861 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     1921 ttctcttatg aagatccctc gacttaatta aggtacccaa ttcgccctat agtgagtcgt
     1981 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     2041 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     2101 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     2161 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     2221 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     2281 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     2341 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     2401 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     2461 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     2521 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     2581 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     2641 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     2701 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     2761 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     2821 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     2881 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     2941 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     3001 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     3061 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     3121 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     3181 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     3241 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     3301 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     3361 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     3421 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     3481 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     3541 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     3601 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     3661 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     3721 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3781 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3841 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3901 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3961 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4021 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4081 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4141 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4201 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4261 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4321 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4381 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     4441 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     4501 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     4561 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     4621 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     4681 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     4741 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     4801 acaaaagctg
//
