LOCUS       sequence-35279-e         647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3859 (=HL 716 + pHL 1305)
FEATURES             Location/Qualifiers
     source          1..647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    344..362
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    369..387
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ccttcgatct cgaactcgtg gccgttcacg gaggccctcc atgtgcacct tgaagcgcat
       61 gaactccttg atgatggcca tgttatcctc ctcgcccttg ctcaccattt tttttcctcc
      121 ttagcatgct ctcacacagc gaggccagat cgggctgctg caatagtgtc cgagtgatgt
      181 cgaacaaccc ttgttgtccg agatcactca tcggtgtata tgacattttg ttcttccagg
      241 aaagcaccgc aaagcggtgc aaaaggtcat tgactaacta taggagattt agaattttat
      301 tagcatatac gcggaagcgg ttcggcgtgt ggtgtcgaca gtatctctat cactgatagg
      361 gatgtcaatc tctatcactg atagggagaa ttcactgctc acaagaaaaa aggcacgtca
      421 tctgacgtgc cttttttatt tgtactaccc tgtacgatta aaggatcctg agcggataca
      481 tatttgaatg tatttagaaa aataaacaaa agagtttgta gaaacgcaaa aaggccatcc
      541 gtcaggatgg cctttctgct taatttgatg cctggcagtt tatggcgggc gtcctgcccg
      601 ccaccctccg ggccgttgct tcgcaacgtt caaatccgct cccggcg
//
