LOCUS       sequence-35280-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3858 (=HL 716 + pHL 1304)
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    318..336
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    343..361
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(503..530)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(622..708)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga
       61 actccttgat gatggccatg ttatcctcct cgcccttgct caccattttt tttcctcctt
      121 agcatgctat catgaacttt cagcgtattc tgactcataa ggtggctcct acccgtgatc
      181 ccttgacgga acattcaagc aaaagcctgg ttccgccgat ttatcgctgc ggcaaataac
      241 gcagcgggtt tacggatttc cccttgtaac gaatttcaaa atgcaagcgt gttgaactgg
      301 ttccggtgtc gacagtatct ctatcactga tagggatgtc aatctctatc actgataggg
      361 agaattcact gctcacaaga aaaaaggcac gtcatctgac gtgccttttt tatttgtact
      421 accctgtacg attaaaggat cctgagcgga tacatatttg aatgtattta gaaaaataaa
      481 caaaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttaatttg
      541 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcgcaac
      601 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
      661 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggcagt
      721 tccctactct cgcatgggga gaccccacac taccatcggc
//
