LOCUS       sequence-352809-       12353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12353)
  AUTHORS   Addis RC, Hsu FC, Wright RL, Dichter MA, Coulter DA, Gearhart JD
  TITLE     Efficient conversion of astrocytes to functional midbrain 
            dopaminergic neurons using a single polycistronic vector.
  JOURNAL   PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub
            2011 Dec 9.
  PUBMED    22174877
REFERENCE   2  (bases 1 to 12353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e28719. doi"; pages: " 
            10.1371/journal.pone.0028719. Epub 2011 Dec 9"
COMMENT     Sequence Label: tetO-ALN
FEATURES             Location/Qualifiers
     source          1..12353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2610..2628,2629..2651,2652..2670,2671..2693,2694..2712,
                     2713..2735,2736..2754,2755..2777,2778..2796,2797..2819,
                     2820..2838,2839..2861,2862..2880)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2913..2951
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2948..2972
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     3051..3070
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3051..3069
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3101..3125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3846..3902
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     RBS             4899..4907
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5028..5081
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(6877..6901)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6927..6968
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6978..6995
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    7045..7633
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7098..7118)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7516..7527)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7636..7652)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7637..7653)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8158..8338
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8364..8381)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8370..8594
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8640..9068
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8727..8746)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8937..8958
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9077..9097)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9082..9411
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9262..9397
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9324..9343
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9459..9506
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9525..9899
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10029..10150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10066..10085)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10120..10139
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10199..10215)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10199..10215)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10212..10234)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10223..10239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10247..10253,10254..10271,10272..10277))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10292..10313
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10430..10447)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10601..11189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10681..10700)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11360..12151,12152..12220))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11983..12002
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12221..12325)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttatcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2641 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2701 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2761 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2821 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2881 aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata
     2941 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     3001 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaattg taatacgact
     3061 cactataggg agacccaagc tggctaggta agcttgatca acaagtttgt acaaaaaagc
     3121 aggctaccat ggaaagctct gccaagatgg agagcggcgg cgccggccag cagccccagc
     3181 cgcagcccca gcagcccttc ctgccgcccg cagcctgttt ctttgccacg gccgcagccg
     3241 cggcggccgc agccgccgca gcggcagcgc agagcgcgca gcagcagcag cagcagcagc
     3301 agcagcagca gcaggcgccg cagctgagac cggcggccga cggccagccc tcagggggcg
     3361 gtcacaagtc agcgcccaag caagtcaagc gacagcgctc gtcttcgccc gaactgatgc
     3421 gctgcaaacg ccggctcaac ttcagcggct ttggctacag cctgccgcag cagcagccgg
     3481 ccgccgtggc gcgccgcaac gagcgcgagc gcaaccgcgt caagttggtc aacctgggct
     3541 ttgccaccct tcgggagcac gtccccaacg gcgcggccaa caagaagatg agtaaggtgg
     3601 agacactgcg ctcggcggtc gagtacatcc gcgcgctgca gcagctgctg gacgagcatg
     3661 acgcggtgag cgccgccttc caggcaggcg tcctgtcgcc caccatctcc cccaactact
     3721 ccaacgactt gaactccatg gccggctcgc cggtctcatc ctactcgtcg gacgagggct
     3781 cttacgaccc gctcagcccc gaggagcagg agcttctcga cttcaccaac tggttcggat
     3841 ccggtgccac gaacttctct ctgttaaagc aagcaggaga tgttgaagaa aaccccgggc
     3901 ctatgttgga cggcatcaag atggaggagc acgccctgcg ccccgggccc gccactctgg
     3961 gggtgctgct gggctccgac tgcccgcatc ccgccgtctg cgagggctgc cagcggccca
     4021 tctccgaccg cttcctgatg cgagtcaacg agtcgtcctg gcacgaggag tgtttgcagt
     4081 gcgcggcgtg tcagcaagcc ctcaccacca gctgctactt ccgggatcgg aagctgtact
     4141 gcaaacaaga ctaccaacag ctcttcgcgg ccaagtgcag cggctgcatg gagaagatcg
     4201 cccccaccga gttcgtgatg cgggcgctgg agtgcgtgta ccacctgggc tgcttctgct
     4261 gctgcgtgtg tgagcggcag ctacgcaagg gcgacgaatt cgtgctcaag gagggccagc
     4321 tgctgtgcaa gggtgactac gagaaggaga aggacctgct cagctccgtg agccccgacg
     4381 agtccgactc cgtgaagagc gaggatgaag atggggacat gaagccggcc aaggggcagg
     4441 gtagtcagag caagggcagc ggggatgacg ggaaggaccc gcggaggccc aagcgacccc
     4501 ggaccatcct caccacgcag cagcgaagag ccttcaaggc ctccttcgag gtctcgtcca
     4561 agccttgccg aaaggtccga gagacactgg cagctgagac gggcctcagt gtgcgcgtgg
     4621 tccaggtctg gtttcagaac caaagagcaa agatgaagaa gctggcgcgg cggcaccagc
     4681 agcagcagga gcagcagaac tcccagcggc tgggccagga ggtcctgtcc agccgcatgg
     4741 agggcatgat ggcttcctac acgccgctgg ccccaccaca gcagcagatc gtggccatgg
     4801 aacagagccc ctacggcagc agcgacccct tccagcaggg cctcacgccg ccccaaatgc
     4861 cagggaacga ctccatcttc catgacatcg acagcgatac ctccttaacc agcctcagcg
     4921 actgcttcct cggctcctca gacgtgggct ccctgcaggc ccgcgtgggg aaccccatcg
     4981 accggctcta ctccatgcag agttcctact tcgcctccgg atccggagag ggcagaggaa
     5041 gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgccttgt gttcaggcgc
     5101 agtatgggtc ctcgcctcaa ggagccagcc ccgcttctca gagctacagt taccactctt
     5161 cgggagaata cagctccgat ttcttaactc cagagtttgt caagtttagc atggacctca
     5221 ccaacactga aatcactgcc accacttctc tccccagctt cagtaccttt atggacaact
     5281 acagcacagg ctacgacgtc aagccacctt gcttgtacca aatgcccctg tccggacagc
     5341 agtcctccat taaggtagaa gacattcaga tgcacaacta ccagcaacac agccacctgc
     5401 ccccccagtc tgaggagatg atgccgcact ccgggtcggt ttactacaag ccctcctcgc
     5461 ccccgacgcc caccaccccg ggcttccagg tgcagcacag ccccatgtgg gacgacccgg
     5521 gatctctcca caacttccac cagaactacg tggccactac gcacatgatc gagcagagga
     5581 aaacgccagt ctcccgcctc tccctcttct cctttaagca atcgccccct ggcaccccgg
     5641 tgtctagttg ccagatgcgc ttcgacgggc ccctgcacgt ccccatgaac ccggagcccg
     5701 ccggcagcca ccacgtggtg gacgggcaga ccttcgctgt gcccaacccc attcgcaagc
     5761 ccgcgtccat gggcttcccg ggcctgcaga tcggccacgc gtctcagctg ctcgacacgc
     5821 aggtgccctc accgccgtcg cggggctccc cctccaacga ggggctgtgc gctgtgtgtg
     5881 gggacaacgc ggcctgccaa cactacggcg tgcgcacctg tgagggctgc aaaggcttct
     5941 ttaagcgcac agtgcaaaaa aatgcaaaat acgtgtgttt agcaaataaa aactgcccag
     6001 tggacaagcg tcgccggaat cgctgtcagt actgccgatt tcagaagtgc ctggctgttg
     6061 ggatggtcaa agaagtggtt cgcacagaca gtttaaaagg ccggagaggt cgtttgccct
     6121 cgaaaccgaa gagcccacag gagccctctc ccccttcgcc cccggtgagt ctgatcagtg
     6181 ccctcgtcag ggcccatgtc gactccaacc cggctatgac cagcctggac tattccaggt
     6241 tccaggcgaa ccctgactat caaatgagtg gagatgacac ccagcatatc cagcaattct
     6301 atgatctcct gactggctcc atggagatca tccggggctg ggcagagaag atccctggct
     6361 tcgcagacct gcccaaagcc gaccaagacc tgctttttga atcagctttc ttagaactgt
     6421 ttgtccttcg attagcatac aggtccaacc cagtggaggg taaactcatc ttttgcaatg
     6481 gggtggtctt gcacaggttg caatgcgttc gtggctttgg ggaatggatt gattccattg
     6541 ttgaattctc ctccaacttg cagaatatga acatcgacat ttctgccttc tcctgcattg
     6601 ctgccctggc tatggtcaca gagagacacg ggctcaagga acccaagaga gtggaagaac
     6661 tgcaaaacaa gattgtaaat tgtctcaaag accacgtgac tttcaacaat ggggggttga
     6721 accgccccaa ttatttgtcc aaactgttgg ggaagctccc agaacttcgt accctttgca
     6781 cacaggggct acagcgcatt ttctacctga aattggaaga cttggtgcca ccgccagcaa
     6841 taattgacaa acttttcctg gacactttac ctttctaccc agctttcttg tacaaagtgg
     6901 ttgatctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg
     6961 attctacgcg taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct
     7021 caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     7081 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7141 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7201 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7261 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7321 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7381 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     7441 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7501 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7561 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7621 gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca
     7681 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     7741 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     7801 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     7861 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
     7921 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
     7981 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
     8041 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
     8101 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg
     8161 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8221 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8281 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
     8341 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     8401 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     8461 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     8521 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     8581 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     8641 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     8701 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     8761 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     8821 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8881 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     8941 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     9001 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     9061 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     9121 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     9181 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     9241 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     9301 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     9361 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     9421 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     9481 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
     9541 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
     9601 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
     9661 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
     9721 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
     9781 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
     9841 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
     9901 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     9961 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
    10021 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    10081 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    10141 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
    10201 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    10261 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    10321 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    10381 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    10441 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    10501 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    10561 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    10621 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    10681 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    10741 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    10801 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    10861 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    10921 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    10981 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    11041 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    11101 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    11161 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    11221 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    11281 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    11341 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    11401 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    11461 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    11521 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    11581 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    11641 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    11701 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    11761 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    11821 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    11881 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    11941 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    12001 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    12061 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    12121 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    12181 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    12241 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    12301 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac
//
