LOCUS       sequence-35281-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3724 (=HL 716 + pHL 991)
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    245..263
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(284..302)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        370..474
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(693..712)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tttacgcatg ctcgcggtac ctttaatcag gttgttcagg ttagtgacat aagtctgatg
       61 gtgcttgccg tagtgatact cgatggtttc cgcagaaatg tgcggtgcca gagcatcttt
      121 agcatatggt agtgcaggta attcgaatga cattgctact ctcctttatt attaatttgc
      181 atacgtacaa tagccttatt gtgcgtatgg tcgacagtct ctctatcact gatagggatg
      241 tcaatctcta tcactgatag ggactcgagg tgaagacgaa agggcctcgt gatacgccta
      301 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
      361 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      421 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      481 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      541 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      601 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      661 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      721 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
      781 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
      841 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
      901 ccgaaggagc taaccgcttt
//
