LOCUS       sequence-35282-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3723 (=HL 716 + pHL 489)
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    202..220
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    227..245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(266..284)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        352..456
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(675..694)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttctccttta ctatcatgaa ctttcagcgt attctgactc ataaggtggc tcctacccgt
       61 gatcccttga cggaacattc aagcaaaagc ctggttccgc cgatttatcg ctgcggcaaa
      121 taacgcagcg ggtttacgga tttccccttg taacgaattt caaaatgcaa gcgtgttgaa
      181 ctggttccgg tgtcgacagt atctctatca ctgataggga tgtcaatctc tatcactgat
      241 agggactcga ggtgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
      301 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
      361 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
      421 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
      481 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
      541 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
      601 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
      661 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
      721 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
      781 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
      841 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
      901 tttttgcaca acatggggga tcatgtaact cgccttgatc gttg
//
