LOCUS       sequence-352830-        8790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.S.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    205..326
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(242..261)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     296..315
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      366..501
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(521..540)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(522..540)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(550..567)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(550..566)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(559..581)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      708..1163
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(795..814)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1005..1026
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1189..1293
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1294..1362,1363..2154)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1512..1531)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2325..2913
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2814..2833
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3067..3084
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3201..3222
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3237..3242,3243..3260,3261..3267)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3275..3291
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3280..3302
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3299..3315
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3299..3315
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3334..3354
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3336..3354
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3382..3608
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3609..3789
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3836..3961
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4454..4687
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4872..4916
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5214..5462
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5214..5234
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5385..5404
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5481..5556
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    5604..5721
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5861..7039
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6092..7030
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6987..7007
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7088..7097
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7136..7180
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             7199..7240
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7250..7957
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7395..7413)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7663..7682
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    8027..8615
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8080..8100)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8498..8509)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             join(8690..8790,1..133)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
       61 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
      121 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
      181 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
      241 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      301 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
      361 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
      421 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
      481 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
      541 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      601 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      661 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
      721 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      781 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      841 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      901 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
      961 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     1021 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     1081 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     1141 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     1201 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     1261 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     1321 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     1381 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1441 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     1501 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     1561 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1621 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1681 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1741 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1801 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     1861 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     1921 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     1981 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     2041 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     2101 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     2161 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     2221 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     2281 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2341 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2401 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2461 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2521 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2581 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2641 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2701 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2761 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2821 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2881 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2941 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3001 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3061 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     3121 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     3181 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     3241 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     3301 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     3361 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     3421 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tggcgattgg
     3481 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     3541 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     3601 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     3661 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     3721 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     3781 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     3841 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     3901 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     3961 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     4021 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     4081 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     4141 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     4201 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     4261 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     4321 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     4381 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     4441 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     4501 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     4561 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     4621 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     4681 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     4741 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     4801 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     4861 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     4921 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     4981 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     5041 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     5101 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     5161 gatctcgacg gtatcgatca cgagactagt atcgagcggc cgcccccttc accgagggcc
     5221 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg
     5281 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
     5341 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
     5401 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
     5461 ccgggtcttc gagaagacct gttttagagc tagaaatagc aagttaaaat aaggctagtc
     5521 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttaattctc gacctcgaga
     5581 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     5641 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     5701 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg
     5761 cggctcgagg cgataagctt tgcaaagatg gataaagttt taaacagaga ggaatctttg
     5821 cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg
     5881 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     5941 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     6001 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     6061 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     6121 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     6181 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     6241 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     6301 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     6361 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     6421 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     6481 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     6541 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     6601 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     6661 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     6721 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     6781 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     6841 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     6901 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     6961 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     7021 tccatttcag gtgtcgtgag gaattctgca gtcgatcgac ggtaccgcgg gcccgggatc
     7081 cacggtcgcc accatgggcg acacctatcg gtacattgga ggaggcgggg gagggaagga
     7141 gaccgccgcc gccaagttcg agaggcagca catggacagc ggaggaggcg ggggaggggg
     7201 caaaccaatt ccaaacccac tgctgggact ggattccaca ggcatgctga tggtgagcaa
     7261 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     7321 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     7381 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     7441 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     7501 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     7561 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     7621 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     7681 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     7741 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     7801 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     7861 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     7921 ccgccactcc accggcggca tggacgagct gtacaagaaa cggccagccg aggatatgga
     7981 ggaagaacaa gcctttaaac gatctcgata aagcggccgc gtcgacaatc aacctctgga
     8041 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     8101 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     8161 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     8221 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     8281 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     8341 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     8401 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     8461 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     8521 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg catcttcgcc ttcgccctca
     8581 gacgagtcgg atctcccttt gggccgcctc cccgcctggg tacctttaag accaatgact
     8641 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     8701 attcactccc aacgaagata agatctgctt tttgcttgta ctgggtctct ctggttagac
     8761 cagatctgag cctgggagct ctctggctaa
//
