LOCUS       sequence-352833-        8778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_AU1.VSVg.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        70..174
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(175..243,244..1035)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(393..412)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1206..1794
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1695..1714
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1948..1965
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2082..2103
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2118..2123,2124..2141,2142..2148)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2156..2172
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2161..2183
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2180..2196
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2180..2196
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2215..2235
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2217..2235
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2263..2489
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2490..2670
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2717..2842
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3335..3568
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3753..3797
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4095..4343
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4095..4115
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4266..4285
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4362..4437
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4485..4602
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4742..5920
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4973..5911
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5868..5888
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5969..5978
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6017..6049
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             6068..6109
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6119..6826
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6264..6282)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6532..6551
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6896..7484
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6949..6969)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7367..7378)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7559..7792
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7864..7985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7901..7920)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7955..7974
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8025..8160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8180..8199)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8181..8199)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8209..8226)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8209..8225)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8218..8240)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8367..8778,1..44)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8454..8473)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8664..8685
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
       61 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      121 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      181 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      241 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      301 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      361 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      421 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
      481 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
      541 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
      601 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
      661 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
      721 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
      781 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
      841 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
      901 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
      961 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1021 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1081 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1141 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1201 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1261 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1321 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1381 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1441 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1501 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1561 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     1621 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     1681 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     1741 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     1801 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     1861 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     1921 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     1981 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2041 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     2101 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     2161 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     2221 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     2281 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     2341 agcaccgtgc atggcgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     2401 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     2461 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     2521 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     2581 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     2641 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     2701 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     2761 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     2821 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     2881 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     2941 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     3001 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     3061 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     3121 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     3181 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     3241 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     3301 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     3361 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     3421 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     3481 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     3541 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     3601 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     3661 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     3721 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     3781 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     3841 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     3901 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     3961 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     4021 gatccattcg attagtgaac ggatctcgac ggtatcgatc acgagactag tatcgagcgg
     4081 ccgccccctt caccgagggc ctatttccca tgattccttc atatttgcat atacgataca
     4141 aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa
     4201 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
     4261 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
     4321 tcttgtggaa aggacgaaac accgggtctt cgagaagacc tgttttagag ctagaaatag
     4381 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     4441 ttttaattct cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     4501 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     4561 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     4621 agcagagatc cactttggcc gcggctcgag gcgataagct ttgcaaagat ggataaagtt
     4681 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
     4741 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     4801 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     4861 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     4921 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
     4981 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
     5041 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
     5101 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
     5161 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
     5221 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
     5281 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
     5341 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
     5401 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
     5461 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     5521 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     5581 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     5641 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     5701 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     5761 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     5821 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     5881 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggaattctgc agtcgatcga
     5941 cggtaccgcg ggcccgggat ccacggtcgc caccatgggc gacacctatc ggtacattgg
     6001 aggaggcggg ggagggtata cagatatcga aatgaataga ctcggaaagg gaggaggcgg
     6061 gggagggggc aaaccaattc caaacccact gctgggactg gattccacag gcatgctgat
     6121 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     6181 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     6241 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     6301 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     6361 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     6421 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     6481 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     6541 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     6601 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     6661 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     6721 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     6781 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagaaac ggccagccga
     6841 ggatatggag gaagaacaag cctttaaacg atctcgataa agcggccgcg tcgacaatca
     6901 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     6961 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7021 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7081 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     7141 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     7201 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     7261 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     7321 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     7381 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc atcttcgcct
     7441 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgggt acctttaaga
     7501 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7561 gaagggctaa ttcactccca acgaagataa gatctgcttt ttgcttgtac tgggtctctc
     7621 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7681 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7741 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     7801 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     7861 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7921 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7981 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     8041 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     8101 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     8161 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     8221 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     8281 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     8341 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     8401 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     8461 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8521 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8581 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8641 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8701 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     8761 aaatgagctg atttaaca
//
