LOCUS       sequence-352835-        8781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_C.HA.HSV_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        203..451
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     203..223
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     374..393
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        470..545
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    593..710
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        850..2028
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1081..2019
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1976..1996
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2077..2086
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2089..2124
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             2143..2169
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2188..2220
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             2230..2937
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2375..2393)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2643..2662
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3007..3595
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3060..3080)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3478..3489)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3670..3903
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3975..4096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4012..4031)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4066..4085
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4136..4271
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4291..4310)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4292..4310)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4320..4337)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4320..4336)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4329..4351)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4478..4933
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4565..4584)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4775..4796
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4959..5063
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5064..5132,5133..5924)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5282..5301)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6095..6683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6584..6603
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6837..6854
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6971..6992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7007..7012,7013..7030,7031..7037)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7045..7061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7050..7072
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7069..7085
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7069..7085
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7104..7124
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7106..7124
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7152..7378
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7379..7559
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7606..7731
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8224..8457
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8642..8686
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
       61 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
      121 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac gagactagta
      181 tcgagcggcc gcccccttca ccgagggcct atttcccatg attccttcat atttgcatat
      241 acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt
      301 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
      361 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
      421 tttatatatc ttgtggaaag gacgaaacac cgggtcttcg agaagacctg ttttagagct
      481 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
      541 ggtgcttttt ttaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa
      601 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      661 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
      721 acagggacag cagagatcca ctttggccgc ggctcgaggc gataagcttt gcaaagatgg
      781 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga
      841 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
      901 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
      961 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     1021 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     1081 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     1141 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
     1201 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
     1261 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
     1321 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
     1381 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
     1441 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
     1501 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     1561 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     1621 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     1681 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     1741 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     1801 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     1861 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     1921 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     1981 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg aattctgcag
     2041 tcgatcgacg gtaccgcggg cccgggatcc acggtcgcca ccatgggcga ggaccaggtg
     2101 gaccccaggc tgatcgacgg caagggagga ggcgggggag ggtacccata cgatgtgcca
     2161 gattacgctg gaggaggcgg gggagggcag cccgagctgg cccccgagga ccccgaggac
     2221 ggcatgctga tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     2281 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     2341 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     2401 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     2461 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     2521 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     2581 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     2641 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     2701 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     2761 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     2821 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     2881 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagaaa
     2941 cggccagccg aggatatgga ggaagaacaa gcctttaaac gatctcgata aagcggccgc
     3001 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3061 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3121 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3181 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3241 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3301 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3361 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     3421 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3481 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3541 catcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggg
     3601 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     3661 aggggggact ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta
     3721 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3781 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     3841 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     3901 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     3961 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4021 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4081 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     4141 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4201 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     4261 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     4321 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     4381 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     4441 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     4501 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4561 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4621 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4681 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4741 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4801 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4861 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4921 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4981 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5041 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5101 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5161 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5221 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5281 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5341 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5401 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5461 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5521 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5581 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5641 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5701 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5761 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5821 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5881 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5941 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6001 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6061 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6121 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6181 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6241 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6301 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6361 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6421 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6481 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6541 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6601 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6661 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6721 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6781 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6841 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     6901 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     6961 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7021 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     7081 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     7141 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     7201 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tggcgattgg tggaagtaag
     7261 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     7321 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     7381 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7441 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7501 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7561 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     7621 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     7681 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     7741 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     7801 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     7861 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     7921 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     7981 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     8041 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     8101 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     8161 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     8221 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     8281 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     8341 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     8401 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     8461 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     8521 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     8581 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     8641 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     8701 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     8761 tttaagaata gtttttgctg t
//
