LOCUS       sequence-352837-        8775 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_C.HA.NWS_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    215..336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(252..271)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     306..325
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      376..511
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(531..550)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(532..550)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(560..577)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(560..576)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(569..591)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      718..1173
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(805..824)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1015..1036
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1199..1303
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1304..1372,1373..2164)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1522..1541)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2335..2923
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2824..2843
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3077..3094
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3211..3232
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3247..3252,3253..3270,3271..3277)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3285..3301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3290..3312
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3309..3325
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3309..3325
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3344..3364
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3346..3364
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3392..3618
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3619..3799
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3846..3971
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4464..4697
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4882..4926
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5224..5472
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5224..5244
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5395..5414
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5491..5566
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    5614..5731
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5871..7049
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6102..7040
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6997..7017
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7098..7107
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7110..7145
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             7164..7190
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7212..7235
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7245..7952
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7390..7408)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7658..7677
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    8022..8610
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8075..8095)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8493..8504)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             join(8685..8775,1..143)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
       61 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      121 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
      181 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
      241 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
      301 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
      361 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
      421 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
      481 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
      541 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
      601 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
      661 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
      721 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      781 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
      841 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
      901 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
      961 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     1021 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     1081 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     1141 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     1201 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     1261 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     1321 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     1381 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     1441 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     1501 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     1561 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     1621 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     1681 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1741 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1801 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1861 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1921 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1981 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     2041 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     2101 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     2161 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     2221 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     2281 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2341 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2401 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2461 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     2521 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2581 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2641 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2701 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2761 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2821 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2881 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2941 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     3001 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     3061 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     3121 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     3181 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     3241 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     3301 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     3361 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     3421 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     3481 tggcgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     3541 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     3601 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     3661 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     3721 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     3781 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     3841 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     3901 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     3961 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     4021 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     4081 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     4141 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     4201 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     4261 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     4321 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     4381 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     4441 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     4501 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     4561 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     4621 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     4681 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     4741 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     4801 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     4861 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     4921 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     4981 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     5041 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     5101 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     5161 ttagtgaacg gatctcgacg gtatcgatca cgagactagt atcgagcggc cgcccccttc
     5221 accgagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     5281 gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     5341 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     5401 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     5461 ggacgaaaca ccgggtcttc gagaagacct gttttagagc tagaaatagc aagttaaaat
     5521 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttaattctc
     5581 gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     5641 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     5701 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     5761 actttggccg cggctcgagg cgataagctt tgcaaagatg gataaagttt taaacagaga
     5821 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
     5881 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     5941 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     6001 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     6061 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
     6121 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
     6181 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
     6241 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
     6301 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
     6361 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
     6421 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
     6481 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     6541 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     6601 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     6661 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     6721 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     6781 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     6841 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     6901 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     6961 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     7021 gtttttttct tccatttcag gtgtcgtgag gaattctgca gtcgatcgac ggtaccgcgg
     7081 gcccgggatc cacggtcgcc accatgggcg aggaccaggt ggaccccagg ctgatcgacg
     7141 gcaagggagg aggcggggga gggtacccat acgatgtgcc agattacgct ggaggaggcg
     7201 ggggagggaa ctggtcccac ccccaattcg agaaaggcat gctgatggtg agcaagggcg
     7261 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     7321 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     7381 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     7441 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     7501 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     7561 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     7621 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     7681 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     7741 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     7801 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     7861 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     7921 actccaccgg cggcatggac gagctgtaca agaaacggcc agccgaggat atggaggaag
     7981 aacaagcctt taaacgatct cgataaagcg gccgcgtcga caatcaacct ctggattaca
     8041 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     8101 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     8161 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     8221 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     8281 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     8341 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     8401 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     8461 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     8521 cccgcggcct gctgccggct ctgcggcctc ttccgcatct tcgccttcgc cctcagacga
     8581 gtcggatctc cctttgggcc gcctccccgc ctgggtacct ttaagaccaa tgacttacaa
     8641 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     8701 ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     8761 ctgagcctgg gagct
//
