LOCUS       sequence-35284-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3619 (=HL 716 + pHL 1228)
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..20)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     RBS             127..138
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    282..300
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    307..325
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(392..419)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(511..597)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     750..767
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 cctcgccctc gcccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc
       61 cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat
      121 cgtacgtttc tcctctttaa taagcttagc gtctctgaag tgaatcgttg aatgcacaat
      181 aaaaaaatcc cgaccctgag ggggtcggga tgaaacttgc ttaagcaaga agcacttaaa
      241 aaattcgtta caccaggaaa tctgatgtgt tcccgggagt atctctatca ctgataggga
      301 tgtcaatctc tatcactgat agggaggatc ctgagcggat acatatttga atgtatttag
      361 aaaaataaac aaaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
      421 cttaatttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc tgggccgttg
      481 cttcgcaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
      541 caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg
      601 cctggcagtt ccctactctc gcatggggag accccacact accatcggcg ctacggcgtt
      661 tcacttctga gttcggcatg gggtcaggtg ggaccaccgc gctactgccg ccaggcaaat
      721 tctgttttat cagaccgctt ctgcgttctg atttaatctg tatcaggctg aaaat
//
