LOCUS       sequence-352840-        8793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_C.HA.S_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        120..224
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(225..293,294..1085)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(443..462)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1256..1844
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1745..1764
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1998..2015
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2132..2153
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2168..2173,2174..2191,2192..2198)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2206..2222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2211..2233
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2230..2246
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2230..2246
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2265..2285
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2267..2285
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2313..2539
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2540..2720
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2767..2892
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3385..3618
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3803..3847
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4145..4393
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4145..4165
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4316..4335
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4412..4487
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4535..4652
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4792..5970
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5023..5961
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5918..5938
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6019..6028
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6031..6066
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6085..6111
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6130..6174
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             6184..6891
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6329..6347)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6597..6616
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6961..7549
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7014..7034)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7432..7443)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7624..7857
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7929..8050
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7966..7985)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8020..8039
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8090..8225
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8245..8264)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8246..8264)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8274..8291)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8274..8290)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8283..8305)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8432..8793,1..94)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8519..8538)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8729..8750
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
       61 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
      121 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      181 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      241 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      301 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
      361 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
      421 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
      481 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
      541 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
      601 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
      661 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
      721 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
      781 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
      841 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
      901 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
      961 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     1021 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     1081 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     1141 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     1201 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1261 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1321 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1381 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     1441 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1501 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1561 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1621 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1681 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1741 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1801 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     1861 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     1921 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     1981 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     2041 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     2101 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     2161 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     2221 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     2281 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     2341 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     2401 atggcgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     2461 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     2521 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     2581 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     2641 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     2701 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     2761 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     2821 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     2881 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     2941 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     3001 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     3061 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     3121 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     3181 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     3241 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     3301 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     3361 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     3421 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     3481 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     3541 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     3601 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     3661 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     3721 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     3781 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     3841 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     3901 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     3961 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     4021 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     4081 attagtgaac ggatctcgac ggtatcgatc acgagactag tatcgagcgg ccgccccctt
     4141 caccgagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag
     4201 agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
     4261 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
     4321 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
     4381 aggacgaaac accgggtctt cgagaagacc tgttttagag ctagaaatag caagttaaaa
     4441 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttaattct
     4501 cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     4561 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     4621 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     4681 cactttggcc gcggctcgag gcgataagct ttgcaaagat ggataaagtt ttaaacagag
     4741 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt
     4801 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     4861 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     4921 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     4981 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
     5041 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
     5101 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
     5161 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
     5221 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
     5281 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
     5341 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
     5401 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
     5461 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
     5521 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     5581 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     5641 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     5701 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     5761 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     5821 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     5881 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     5941 agtttttttc ttccatttca ggtgtcgtga ggaattctgc agtcgatcga cggtaccgcg
     6001 ggcccgggat ccacggtcgc caccatgggc gaggaccagg tggaccccag gctgatcgac
     6061 ggcaagggag gaggcggggg agggtaccca tacgatgtgc cagattacgc tggaggaggc
     6121 gggggaggga aggagaccgc cgccgccaag ttcgagaggc agcacatgga cagcggcatg
     6181 ctgatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     6241 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     6301 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     6361 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     6421 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     6481 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     6541 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     6601 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     6661 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     6721 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     6781 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     6841 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gaaacggcca
     6901 gccgaggata tggaggaaga acaagccttt aaacgatctc gataaagcgg ccgcgtcgac
     6961 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7021 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7081 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7141 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7201 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7261 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7321 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     7381 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7441 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcatctt
     7501 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgggtacctt
     7561 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     7621 gactggaagg gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt
     7681 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7741 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     7801 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     7861 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     7921 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7981 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8041 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     8101 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     8161 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     8221 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     8281 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     8341 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     8401 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     8461 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     8521 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     8581 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     8641 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     8701 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     8761 aaccctatct cggtctattc ttttgattta taa
//
