LOCUS       sequence-352841-        8781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_C.HA.VSVg_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        79..183
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(184..252,253..1044)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(402..421)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1215..1803
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1704..1723
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1957..1974
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2091..2112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2127..2132,2133..2150,2151..2157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2165..2181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2170..2192
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2189..2205
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2189..2205
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2224..2244
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2226..2244
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2272..2498
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2499..2679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2726..2851
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3344..3577
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3762..3806
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4104..4352
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4104..4124
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4275..4294
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4371..4446
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4494..4611
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4751..5929
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4982..5920
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5877..5897
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5978..5987
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5990..6025
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6044..6070
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6089..6121
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             6131..6838
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6276..6294)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6544..6563
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6908..7496
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6961..6981)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7379..7390)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7571..7804
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7876..7997
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7913..7932)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7967..7986
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8037..8172
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8192..8211)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8193..8211)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8221..8238)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8221..8237)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8230..8252)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8379..8781,1..53)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8466..8485)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8676..8697
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
       61 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      121 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      181 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      241 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      301 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      361 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      421 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
      481 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
      541 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
      601 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
      661 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
      721 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
      781 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
      841 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
      901 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
      961 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1021 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1081 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1141 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1201 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1261 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1321 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1381 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1441 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1501 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     1561 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     1621 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     1681 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     1741 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     1801 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     1861 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     1921 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     1981 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     2041 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     2101 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     2161 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     2221 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     2281 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     2341 gagagaaaaa gcaccgtgca tggcgattgg tggaagtaag gtggtacgat cgtgccttat
     2401 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     2461 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     2521 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     2581 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     2641 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     2701 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     2761 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     2821 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     2881 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     2941 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     3001 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     3061 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     3121 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     3181 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     3241 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     3301 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     3361 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     3421 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     3481 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     3541 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     3601 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     3661 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     3721 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     3781 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     3841 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     3901 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     3961 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     4021 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagt
     4081 atcgagcggc cgcccccttc accgagggcc tatttcccat gattccttca tatttgcata
     4141 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
     4201 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
     4261 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
     4321 ctttatatat cttgtggaaa ggacgaaaca ccgggtcttc gagaagacct gttttagagc
     4381 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     4441 cggtgctttt tttaattctc gacctcgaga caaatggcag tattcatcca caattttaaa
     4501 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     4561 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     4621 tacagggaca gcagagatcc actttggccg cggctcgagg cgataagctt tgcaaagatg
     4681 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
     4741 agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     4801 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     4861 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     4921 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     4981 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     5041 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
     5101 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
     5161 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
     5221 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
     5281 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
     5341 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     5401 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     5461 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     5521 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     5581 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     5641 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     5701 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     5761 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     5821 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     5881 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattctgca
     5941 gtcgatcgac ggtaccgcgg gcccgggatc cacggtcgcc accatgggcg aggaccaggt
     6001 ggaccccagg ctgatcgacg gcaagggagg aggcggggga gggtacccat acgatgtgcc
     6061 agattacgct ggaggaggcg ggggagggta tacagatatc gaaatgaata gactcggaaa
     6121 gggcatgctg atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     6181 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     6241 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     6301 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     6361 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     6421 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     6481 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     6541 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     6601 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     6661 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     6721 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     6781 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagaa
     6841 acggccagcc gaggatatgg aggaagaaca agcctttaaa cgatctcgat aaagcggccg
     6901 cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6961 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     7021 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7081 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7141 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7201 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7261 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     7321 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7381 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7441 gcatcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     7501 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     7561 aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct ttttgcttgt
     7621 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7681 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7741 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7801 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7861 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7921 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7981 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     8041 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8101 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     8161 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     8221 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     8281 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     8341 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     8401 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     8461 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     8521 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     8581 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     8641 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     8701 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     8761 ctattggtta aaaaatgagc t
//
