LOCUS       sequence-352843-        8790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_C.HA.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..186
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(187..255,256..1047)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(405..424)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1218..1806
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1707..1726
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1960..1977
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2094..2115
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2130..2135,2136..2153,2154..2160)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2168..2184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2173..2195
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2192..2208
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2192..2208
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2227..2247
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2229..2247
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2275..2501
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2502..2682
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2729..2854
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3347..3580
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3765..3809
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4107..4355
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4107..4127
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4278..4297
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4374..4449
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4497..4614
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4754..5932
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4985..5923
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5880..5900
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5981..5990
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5993..6028
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6047..6073
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6092..6133
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6143..6850
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6288..6306)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6556..6575
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6920..7508
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6973..6993)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7391..7402)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7583..7816
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7888..8009
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7925..7944)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7979..7998
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8049..8184
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8204..8223)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8205..8223)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8233..8250)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8233..8249)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8242..8264)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8391..8790,1..56)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8478..8497)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8688..8709
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
       61 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      121 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      181 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      241 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      301 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
      361 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      421 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
      481 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
      541 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
      601 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
      661 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
      721 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
      781 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
      841 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
      901 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
      961 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     1021 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     1081 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1141 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1201 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1261 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1321 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1381 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1441 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1501 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1561 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1621 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1681 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1741 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1801 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     1861 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     1921 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     1981 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     2041 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     2101 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     2161 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     2221 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     2281 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     2341 aaggagagaa aaagcaccgt gcatggcgat tggtggaagt aaggtggtac gatcgtgcct
     2401 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     2461 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     2521 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     2581 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     2641 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     2701 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     2761 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     2821 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     2881 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     2941 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     3001 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     3061 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     3121 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     3181 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     3241 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     3301 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     3361 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     3421 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     3481 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     3541 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     3601 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     3661 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     3721 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     3781 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     3841 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     3901 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     3961 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     4021 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga tcacgagact
     4081 agtatcgagc ggccgccccc ttcaccgagg gcctatttcc catgattcct tcatatttgc
     4141 atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga
     4201 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     4261 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     4321 tggctttata tatcttgtgg aaaggacgaa acaccgggtc ttcgagaaga cctgttttag
     4381 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
     4441 agtcggtgct ttttttaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt
     4501 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     4561 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     4621 tattacaggg acagcagaga tccactttgg ccgcggctcg aggcgataag ctttgcaaag
     4681 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
     4741 aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     4801 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     4861 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     4921 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     4981 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     5041 cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     5101 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     5161 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     5221 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     5281 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     5341 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     5401 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     5461 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     5521 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     5581 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     5641 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     5701 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     5761 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     5821 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     5881 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggaattct
     5941 gcagtcgatc gacggtaccg cgggcccggg atccacggtc gccaccatgg gcgaggacca
     6001 ggtggacccc aggctgatcg acggcaaggg aggaggcggg ggagggtacc catacgatgt
     6061 gccagattac gctggaggag gcgggggagg gggcaaacca attccaaacc cactgctggg
     6121 actggattcc acaggcatgc tgatggtgag caagggcgag gaggataaca tggccatcat
     6181 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     6241 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     6301 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     6361 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     6421 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     6481 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     6541 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     6601 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     6661 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     6721 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     6781 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     6841 gctgtacaag aaacggccag ccgaggatat ggaggaagaa caagccttta aacgatctcg
     6901 ataaagcggc cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6961 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     7021 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     7081 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     7141 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     7201 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7261 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     7321 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7381 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7441 gcggcctctt ccgcatcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7501 ctccccgcct gggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7561 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag ataagatctg
     7621 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7681 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7741 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7801 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     7861 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     7921 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7981 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     8041 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8101 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8161 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     8221 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     8281 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     8341 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     8401 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     8461 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     8521 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     8581 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     8641 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     8701 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     8761 gccgatttcg gcctattggt taaaaaatga
//
