LOCUS       sequence-352846-        8781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_C.HSV.NWS_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        113..217
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(218..286,287..1078)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(436..455)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1249..1837
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1738..1757
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1991..2008
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2125..2146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2161..2166,2167..2184,2185..2191)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2199..2215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2204..2226
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2223..2239
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2223..2239
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2258..2278
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2260..2278
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2306..2532
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2533..2713
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2760..2885
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3378..3611
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3796..3840
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4138..4386
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4138..4158
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4309..4328
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4405..4480
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4528..4645
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4785..5963
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5016..5954
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5911..5931
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6012..6021
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6024..6059
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6078..6110
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             6132..6155
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6165..6872
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6310..6328)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6578..6597
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6942..7530
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6995..7015)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7413..7424)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7605..7838
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7910..8031
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7947..7966)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8001..8020
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8071..8206
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8226..8245)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8227..8245)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8255..8272)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8255..8271)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8264..8286)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8413..8781,1..87)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8500..8519)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8710..8731
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
       61 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
      121 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      181 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      241 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      301 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      361 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      421 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
      481 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
      541 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
      601 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
      661 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
      721 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
      781 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
      841 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
      901 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
      961 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     1021 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     1081 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     1141 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1201 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1261 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1321 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1381 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1441 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1501 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1561 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1621 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1681 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1741 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1801 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1861 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1921 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     1981 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     2041 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     2101 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     2161 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     2221 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     2281 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     2341 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatggcga
     2401 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     2461 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     2521 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     2581 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     2641 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     2701 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     2761 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     2821 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     2881 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     2941 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     3001 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     3061 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     3121 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     3181 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     3241 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     3301 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     3361 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     3421 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     3481 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     3541 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     3601 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     3661 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     3721 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     3781 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     3841 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     3901 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     3961 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     4021 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     4081 aacggatctc gacggtatcg atcacgagac tagtatcgag cggccgcccc cttcaccgag
     4141 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
     4201 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
     4261 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
     4321 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     4381 aacaccgggt cttcgagaag acctgtttta gagctagaaa tagcaagtta aaataaggct
     4441 agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttaat tctcgacctc
     4501 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     4561 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     4621 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     4681 gccgcggctc gaggcgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
     4741 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     4801 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     4861 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     4921 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     4981 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     5041 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     5101 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     5161 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     5221 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     5281 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     5341 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     5401 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     5461 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     5521 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     5581 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     5641 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     5701 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     5761 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     5821 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     5881 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     5941 ttcttccatt tcaggtgtcg tgaggaattc tgcagtcgat cgacggtacc gcgggcccgg
     6001 gatccacggt cgccaccatg ggcgaggacc aggtggaccc caggctgatc gacggcaagg
     6061 gaggaggcgg gggagggcag cccgagctgg cccccgagga ccccgaggac ggaggaggcg
     6121 ggggagggaa ctggtcccac ccccaattcg agaaaggcat gctgatggtg agcaagggcg
     6181 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     6241 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     6301 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     6361 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     6421 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     6481 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     6541 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     6601 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     6661 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     6721 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     6781 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     6841 actccaccgg cggcatggac gagctgtaca agaaacggcc agccgaggat atggaggaag
     6901 aacaagcctt taaacgatct cgataaagcg gccgcgtcga caatcaacct ctggattaca
     6961 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     7021 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7081 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7141 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7201 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7261 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7321 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     7381 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7441 cccgcggcct gctgccggct ctgcggcctc ttccgcatct tcgccttcgc cctcagacga
     7501 gtcggatctc cctttgggcc gcctccccgc ctgggtacct ttaagaccaa tgacttacaa
     7561 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     7621 ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     7681 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7741 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7801 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     7861 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     7921 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7981 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8041 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8101 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8161 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     8221 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     8281 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     8341 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     8401 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     8461 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     8521 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     8581 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     8641 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     8701 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     8761 cttttgattt ataagggatt t
//
