LOCUS       sequence-352847-        8799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_C.HSV.Ollas_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        70..174
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(175..243,244..1035)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(393..412)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1206..1794
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1695..1714
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1948..1965
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2082..2103
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2118..2123,2124..2141,2142..2148)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2156..2172
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2161..2183
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2180..2196
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2180..2196
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2215..2235
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2217..2235
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2263..2489
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2490..2670
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2717..2842
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3335..3568
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3753..3797
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4095..4343
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4095..4115
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4266..4285
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4362..4437
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4485..4602
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4742..5920
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4973..5911
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5868..5888
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5969..5978
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5981..6016
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6035..6067
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             6086..6127
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             6140..6847
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6285..6303)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6553..6572
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6917..7505
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6970..6990)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7388..7399)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7580..7813
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7885..8006
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7922..7941)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7976..7995
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8046..8181
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8201..8220)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8202..8220)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8230..8247)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8230..8246)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8239..8261)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8388..8799,1..44)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8475..8494)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8685..8706
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
       61 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      121 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      181 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      241 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      301 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      361 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      421 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
      481 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
      541 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
      601 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
      661 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
      721 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
      781 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
      841 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
      901 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
      961 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1021 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1081 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1141 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1201 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1261 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1321 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1381 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1441 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1501 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1561 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     1621 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     1681 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     1741 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     1801 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     1861 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     1921 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     1981 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2041 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     2101 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     2161 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     2221 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     2281 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     2341 agcaccgtgc atggcgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     2401 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     2461 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     2521 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     2581 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     2641 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     2701 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     2761 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     2821 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     2881 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     2941 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     3001 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     3061 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     3121 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     3181 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     3241 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     3301 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     3361 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     3421 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     3481 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     3541 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     3601 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     3661 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     3721 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     3781 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     3841 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     3901 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     3961 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     4021 gatccattcg attagtgaac ggatctcgac ggtatcgatc acgagactag tatcgagcgg
     4081 ccgccccctt caccgagggc ctatttccca tgattccttc atatttgcat atacgataca
     4141 aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa
     4201 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
     4261 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
     4321 tcttgtggaa aggacgaaac accgggtctt cgagaagacc tgttttagag ctagaaatag
     4381 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     4441 ttttaattct cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     4501 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     4561 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     4621 agcagagatc cactttggcc gcggctcgag gcgataagct ttgcaaagat ggataaagtt
     4681 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
     4741 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     4801 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     4861 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     4921 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
     4981 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
     5041 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
     5101 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
     5161 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
     5221 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
     5281 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
     5341 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
     5401 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
     5461 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
     5521 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
     5581 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
     5641 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
     5701 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
     5761 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
     5821 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
     5881 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggaattctgc agtcgatcga
     5941 cggtaccgcg ggcccgggat ccacggtcgc caccatgggc gaggaccagg tggaccccag
     6001 gctgatcgac ggcaagggag gaggcggggg agggcagccc gagctggccc ccgaggaccc
     6061 cgaggacgga ggaggcgggg gagggagcgg cttcgccaac gagctgggcc ccaggctgat
     6121 gggcaagtgc ggcatgctga tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     6181 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     6241 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     6301 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     6361 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     6421 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     6481 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     6541 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     6601 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     6661 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     6721 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     6781 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     6841 gtacaagaaa cggccagccg aggatatgga ggaagaacaa gcctttaaac gatctcgata
     6901 aagcggccgc gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6961 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7021 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7081 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7141 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7201 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7261 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     7321 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7381 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7441 gcctcttccg catcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7501 cccgcctggg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7561 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata agatctgctt
     7621 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7681 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7741 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7801 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     7861 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     7921 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7981 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     8041 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8101 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8161 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     8221 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     8281 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     8341 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     8401 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8461 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8521 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8581 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8641 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8701 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     8761 gatttcggcc tattggttaa aaaatgagct gatttaaca
//
