LOCUS       sequence-352848-        8799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_C.HSV.S_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        123..227
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(228..296,297..1088)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(446..465)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1259..1847
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1748..1767
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2001..2018
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2135..2156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2171..2176,2177..2194,2195..2201)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2209..2225
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2214..2236
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2233..2249
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2233..2249
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2268..2288
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2270..2288
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2316..2542
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2543..2723
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2770..2895
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3388..3621
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3806..3850
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4148..4396
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4148..4168
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4319..4338
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4415..4490
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4538..4655
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4795..5973
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5026..5964
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5921..5941
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6022..6031
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6034..6069
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6088..6120
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             6139..6183
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             6193..6900
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6338..6356)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6606..6625
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6970..7558
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7023..7043)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7441..7452)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7633..7866
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7938..8059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7975..7994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8029..8048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8099..8234
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8254..8273)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8255..8273)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8283..8300)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8283..8299)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8292..8314)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8441..8799,1..97)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8528..8547)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8738..8759
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
       61 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
      121 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      181 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      241 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      301 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      361 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      421 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      481 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      541 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      601 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      661 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      721 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
      781 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
      841 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
      901 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
      961 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1021 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1081 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1141 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1201 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1261 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1321 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1381 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1441 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttccca gtggctgctg
     1501 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1561 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1621 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1681 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1741 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     1801 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     1861 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     1921 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     1981 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2041 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     2101 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     2161 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     2221 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     2281 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     2341 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     2401 tgcatggcga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     2461 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     2521 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     2581 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     2641 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     2701 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     2761 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     2821 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     2881 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     2941 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     3001 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     3061 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     3121 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     3181 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     3241 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     3301 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     3361 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     3421 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     3481 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     3541 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     3601 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     3661 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     3721 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     3781 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     3841 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     3901 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     3961 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     4021 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     4081 tcgattagtg aacggatctc gacggtatcg atcacgagac tagtatcgag cggccgcccc
     4141 cttcaccgag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
     4201 tagagagata attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
     4261 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
     4321 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
     4381 gaaaggacga aacaccgggt cttcgagaag acctgtttta gagctagaaa tagcaagtta
     4441 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttaat
     4501 tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     4561 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     4621 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     4681 atccactttg gccgcggctc gaggcgataa gctttgcaaa gatggataaa gttttaaaca
     4741 gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc
     4801 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     4861 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     4921 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     4981 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
     5041 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
     5101 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
     5161 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     5221 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     5281 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     5341 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
     5401 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     5461 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     5521 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     5581 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
     5641 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     5701 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     5761 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     5821 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     5881 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     5941 caaagttttt ttcttccatt tcaggtgtcg tgaggaattc tgcagtcgat cgacggtacc
     6001 gcgggcccgg gatccacggt cgccaccatg ggcgaggacc aggtggaccc caggctgatc
     6061 gacggcaagg gaggaggcgg gggagggcag cccgagctgg cccccgagga ccccgaggac
     6121 ggaggaggcg ggggagggaa ggagaccgcc gccgccaagt tcgagaggca gcacatggac
     6181 agcggcatgc tgatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     6241 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     6301 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     6361 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     6421 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     6481 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     6541 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     6601 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     6661 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     6721 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     6781 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     6841 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     6901 aaacggccag ccgaggatat ggaggaagaa caagccttta aacgatctcg ataaagcggc
     6961 cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     7021 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     7081 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     7141 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     7201 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7261 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     7321 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     7381 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7441 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7501 ccgcatcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     7561 gggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7621 aaaagggggg actggaaggg ctaattcact cccaacgaag ataagatctg ctttttgctt
     7681 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7741 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7801 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7861 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7921 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7981 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8041 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     8101 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8161 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8221 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     8281 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     8341 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     8401 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     8461 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     8521 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     8581 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     8641 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     8701 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     8761 acaacactca accctatctc ggtctattct tttgattta
//
