LOCUS       sequence-352849-        8787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_C.HSV.VSVg_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..202
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(203..271,272..1063)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(421..440)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1234..1822
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1723..1742
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1976..1993
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2110..2131
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2146..2151,2152..2169,2170..2176)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2184..2200
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2189..2211
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2208..2224
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2208..2224
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2243..2263
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2245..2263
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2291..2517
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2518..2698
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2745..2870
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3363..3596
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3781..3825
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4123..4371
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4123..4143
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4294..4313
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4390..4465
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4513..4630
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4770..5948
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5001..5939
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5896..5916
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5997..6006
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6009..6044
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6063..6095
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             6114..6146
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             6156..6863
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6301..6319)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6569..6588
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6933..7521
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6986..7006)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7404..7415)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7596..7829
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7901..8022
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7938..7957)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7992..8011
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8062..8197
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8217..8236)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8218..8236)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8246..8263)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8246..8262)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8255..8277)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8404..8787,1..72)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8491..8510)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8701..8722
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
       61 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      121 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      181 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      241 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      301 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      361 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      421 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      481 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      541 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      601 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      661 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
      721 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
      781 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
      841 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
      901 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
      961 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1021 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1081 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1141 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1201 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1261 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1321 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1381 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1441 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1501 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1561 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1621 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1681 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1741 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     1801 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     1861 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     1921 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     1981 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     2041 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     2101 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     2161 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     2221 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     2281 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     2341 tagcaacatg ccttacaagg agagaaaaag caccgtgcat ggcgattggt ggaagtaagg
     2401 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     2461 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
     2521 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     2581 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     2641 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     2701 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     2761 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     2821 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     2881 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     2941 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     3001 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     3061 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     3121 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     3181 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     3241 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     3301 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     3361 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     3421 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     3481 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     3541 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     3601 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     3661 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     3721 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     3781 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     3841 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     3901 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     3961 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     4021 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     4081 tatcgatcac gagactagta tcgagcggcc gcccccttca ccgagggcct atttcccatg
     4141 attccttcat atttgcatat acgatacaag gctgttagag agataattgg aattaatttg
     4201 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
     4261 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
     4321 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cgggtcttcg
     4381 agaagacctg ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
     4441 tgaaaaagtg gcaccgagtc ggtgcttttt ttaattctcg acctcgagac aaatggcagt
     4501 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     4561 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     4621 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc ggctcgaggc
     4681 gataagcttt gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga
     4741 ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca
     4801 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     4861 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     4921 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     4981 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     5041 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     5101 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     5161 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     5221 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     5281 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     5341 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     5401 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     5461 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     5521 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     5581 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     5641 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     5701 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     5761 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     5821 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     5881 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     5941 tgtcgtgagg aattctgcag tcgatcgacg gtaccgcggg cccgggatcc acggtcgcca
     6001 ccatgggcga ggaccaggtg gaccccaggc tgatcgacgg caagggagga ggcgggggag
     6061 ggcagcccga gctggccccc gaggaccccg aggacggagg aggcggggga gggtatacag
     6121 atatcgaaat gaatagactc ggaaagggca tgctgatggt gagcaagggc gaggaggata
     6181 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     6241 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     6301 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     6361 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     6421 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     6481 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     6541 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     6601 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     6661 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     6721 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     6781 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     6841 gcggcatgga cgagctgtac aagaaacggc cagccgagga tatggaggaa gaacaagcct
     6901 ttaaacgatc tcgataaagc ggccgcgtcg acaatcaacc tctggattac aaaatttgtg
     6961 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7021 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7081 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7141 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7201 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7261 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     7321 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7381 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7441 tgctgccggc tctgcggcct cttccgcatc ttcgccttcg ccctcagacg agtcggatct
     7501 ccctttgggc cgcctccccg cctgggtacc tttaagacca atgacttaca aggcagctgt
     7561 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7621 aagataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7681 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7741 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7801 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     7861 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     7921 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7981 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     8041 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     8101 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     8161 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     8221 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     8281 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     8341 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     8401 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     8461 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     8521 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     8581 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     8641 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     8701 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     8761 tataagggat tttgccgatt tcggcct
//
