LOCUS       sequence-35285-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3545 (=HL 770 + pHL 1213)
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    43..61
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(107..125)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        193..297
                     /gene="bla"
                     /label=AmpR promoter
     CDS             482..860
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(516..535)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcttctcctt tacgcatttt ttttcctcct tagtcgacag tatctctatc actgataggg
       61 atgtcaatct ctatcactga tagggactcg aggtgaagac gaaagggcct cgtgatacgc
      121 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
      181 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      241 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      301 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
      361 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
      421 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
      481 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
      541 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
      601 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
      661 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
      721 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
      781 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
      841 gtagcaatgg caacaacgtt gcgcaaact
//
