LOCUS       sequence-35286-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3612 (=HL 716 + pHL 841)
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             124..135
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    275..293
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    300..318
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(385..412)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(504..590)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     743..760
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg aagccctcca tgtgcacctt
       61 gaagcgcatg aactccttga tgatggccat gttatcctcc tcgcccttgc tcaccatcgt
      121 acgtttctcc tctttaataa gcttgtggat aaattgagaa cgaaagatca aaaaaaaagc
      181 cagcacccgg ctggctaagt aatactggaa gcaatgtgag caatgtcgtg ctttcaggtt
      241 ctccgcgagg gtcttcctga tcgccccggg agtatctcta tcactgatag ggatgtcaat
      301 ctctatcact gatagggagg atcctgagcg gatacatatt tgaatgtatt tagaaaaata
      361 aacaaaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttaatt
      421 tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttggttcgca
      481 acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
      541 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggca
      601 gttccctact ctcgcatggg gagaccccac actaccatcg gcgctacggc gtttcacttc
      661 tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccaggca aattctgttt
      721 tatcagaccg cttctgcgtt ctgatttaat ctgtatcagg ctgaaaatct tctctcatcc
      781 gccaaaacag ccaagctagc ttttatttgt agagctcatc c
//
