LOCUS       sequence-35288-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3450 (=HL 3262 + pHL 1191)
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             125..136
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    306..322
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(374..460)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     613..630
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(716..736)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc atgtgcacct
       61 tgaagcgcat gaactccttg atgatggcca tgttatcctc ctcgcccttg ctcaccatcg
      121 tacgtttctc ctctttaata agctttgagc ctggcttatc gccgggcttt tttatggcaa
      181 aaaaaagcgg atcctggaga tccgcaaaag ttcacgttgg ctttagttat tcgagttgag
      241 aaactctcga aacgggcagt gacttcaagg gttaaaagag gtgccgctcc gtttccggga
      301 gtatcttgtt atccgctcac aatgtcaatg ttatccgctc acatttatgg atcccctagg
      361 tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
      421 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggca gttccctact
      481 ctcgcatggg gagaccccac actaccatcg gcgctacggc gtttcacttc tgagttcggc
      541 atggggtcag gtgggaccac cgcgctactg ccgccaggca aattctgttt tatcagaccg
      601 cttctgcgtt ctgatttaat ctgtatcagg ctgaaaatct tctctcatcc gccaaaacag
      661 ccaagctagc ttttatttgt agagctcatc catgccatgt gtaatcccag cagctgttac
      721 aaactcaaga aggaccatgt ggtctctctt ttcgttggga tctttcgaaa gggcagattg
      781 tgtggacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg g
//
