LOCUS       sequence-352904-        2535 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PfuX7 DNA polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Norholm MH
  TITLE     A mutant Pfu DNA polymerase designed for advanced uracil-excision 
            DNA engineering.
  JOURNAL   BMC Biotechnol. 2010 Mar 16;10:21. doi: 10.1186/1472-6750-10-21.
  PUBMED    20233396
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2010-03-16"; volume: "10:21. doi"; pages: " 
            10.1186/1472-6750-10-21"
COMMENT     Sequence Label: pET-PfuX7
FEATURES             Location/Qualifiers
     source          1..2535
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgattttag atgtggatta cataactgaa gaaggaaaac ctgttattag gctattcaaa
       61 aaagagaacg gaaaatttaa gatagagcat gatagaactt ttagaccata catttacgct
      121 cttctcaggg atgattcaaa gattgaagaa gttaagaaaa taacggggga aaggcatgga
      181 aagattgtga gaattgttga tgtagagaag gttgagaaaa agtttctcgg caagcctatt
      241 accgtgtgga aactttattt ggaacatccc caagatcaac ccactattag agaaaaagtt
      301 agagaacatc cagcagttgt ggacatcttc gaatacgata ttccatttgc aaagagatac
      361 ctcatcgaca aaggcctaat accaatggag ggggaagaag agctaaagat tcttgccttc
      421 gatatagaaa ccctctatca cgaaggagaa gagtttggaa aaggcccaat tataatgatt
      481 agttatgcag atgaaaatga agcaaaggtg attacttgga aaaacataga tcttccatac
      541 gttgaggttg tatcaagcga gagagagatg ataaagagat ttctcaggat tatcagggag
      601 aaggatcctg acattatagt tacttataat ggagactcat tcgacttccc atatttagcg
      661 aaaagggcag aaaaacttgg gattaaatta accattggaa gagatggaag cgagcccaag
      721 atgcagagaa taggcgatat gacggctgta gaagtcaagg gaagaataca tttcgacttg
      781 tatcatgtaa taacaaggac aataaatctc ccaacataca cactagaggc tgtatatgaa
      841 gcaatttttg gaaagccaaa ggagaaggta tacgccgacg agatagcaaa agcctgggaa
      901 agtggagaga accttgagag agttgccaaa tactcgatgg aagatgcaaa ggcaacttat
      961 gaactcggga aagaattcct tccaatggaa attcagcttt caagattagt tggacaacct
     1021 ttatgggatg tttcaaggtc aagcacaggg aaccttgtag agtggttctt acttaggaaa
     1081 gcctacgaaa gaaacgaagt agctccaaac aagccaagtg aagaggagta tcaaagaagg
     1141 ctcagggaga gctacacagg tggattcgtt aaagagccag aaaaggggtt gtgggaaaac
     1201 atagtatacc tagattttag agccctatat ccctcgatta taattaccca caatgtttct
     1261 cccgatactc taaatcttga gggatgcaag aactatgata tcgctcctca agtaggccac
     1321 aagttctgca aggacatccc tggttttata ccaagtctct tgggacattt gttagaggaa
     1381 agacaaaaga ttaagacaaa aatgaaggaa actcaagatc ctatagaaaa aatactcctt
     1441 gactatagac aaaaagcgat aaaactctta gcaaattctt tctacggata ttatggctat
     1501 gcaaaagcaa gatggtactg taaggagtgt gctgagagcg ttactgcctg gggaagaaag
     1561 tacatcgagt tagtatggaa ggagctcgaa gaaaagtttg gatttaaagt cctctacatt
     1621 gacactgatg gtctctatgc aactatccca ggaggagaaa gtgaggaaat aaagaaaaag
     1681 gctctagaat ttgtaaaata cataaattca aagctccctg gactgctaga gcttgaatat
     1741 gaagggtttt ataagagggg attcttcgtt acgaagaaga ggtatgcagt aatagatgaa
     1801 gaaggaaaag tcattactcg tggtttagag atagttagga gagattggag tgaaattgca
     1861 aaagaaactc aagctagagt tttggagaca atactaaaac acggagatgt tgaagaagct
     1921 gtgagaatag taaaagaagt aatacaaaag cttgccaatt atgaaattcc accagagaag
     1981 ctcgcaatat atgagcagat aacaagacca ttacatgagt ataaggcgat aggtcctcac
     2041 gtagctgttg caaagaaact agctgctaaa ggagttaaaa taaagccagg aatggtaatt
     2101 ggatacatag tacttagagg cgatggtcca attagcaata gggcaattct agctgaggaa
     2161 tacgatccca aaaagcacaa gtatgacgca gaatattaca ttgagaacca ggttcttcca
     2221 gcggtactta ggatattgga gggatttgga tacagaaagg aagacctcag ataccaaaag
     2281 acaagacaag tcggcctaac ttcctggctt aacattaaaa aatccggtac cggcggtggc
     2341 ggtgcaaccg taaagttcaa gtacaaaggc gaagaaaaag aggtagacat ctccaagatc
     2401 aagaaagtat ggcgtgtggg caagatgatc tccttcacct acgacgaggg cggtggcaag
     2461 accggccgtg gtgcggtaag cgaaaaggac gcgccgaagg agctgctgca gatgctggag
     2521 aagcagaaaa agtga
//
