LOCUS       sequence-352909-        9368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9368)
  AUTHORS   Sorg G, Stamminger T
  TITLE     Mapping of nuclear localization signals by simultaneous fusion to 
            green fluorescent protein and to beta-galactosidase.
  JOURNAL   Biotechniques. 1999 May . 26(5):858-62.
  PUBMED    10337476
REFERENCE   2  (bases 1 to 9368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 1999 May . 26(5):858-62."
COMMENT     Sequence Label: pHM840
FEATURES             Location/Qualifiers
     source          1..9368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..99)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        84..413
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      264..399
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     326..345
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             480..1274
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(534..553)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1144..1163
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1448..1569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1485..1504)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1539..1558
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1618..1634)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1618..1634)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1631..1653)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1642..1658
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1666..1672,1673..1690,1691..1696))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1711..1732
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1849..1866)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2020..2608)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2100..2119)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2779..3570,3571..3639))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3402..3421
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3640..3744)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3819..3838)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4010..4389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4390..4593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4544..4564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4638..4657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4638..4656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4679..5392
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             5444..5464
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5627..8674
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDVWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(5629..5651)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        complement(8696..8714)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8697..8714)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8734..8751)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8740..8964
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9010..9368,1..70)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9097..9116)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9307..9328
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
       61 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
      121 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
      181 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
      241 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
      301 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
      361 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
      421 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
      481 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
      541 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
      601 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
      661 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
      721 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
      781 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
      841 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
      901 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
      961 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     1021 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     1081 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     1141 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     1201 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     1261 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     1321 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     1381 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     1441 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     1501 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     1561 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     1621 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     1681 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     1741 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     1801 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     1861 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1921 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1981 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2041 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2101 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2161 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2221 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2281 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2341 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2401 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2461 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2521 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2581 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2641 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2701 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2761 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2821 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2881 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     2941 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3001 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3061 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3121 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3181 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3241 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3301 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3361 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3421 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3481 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     3541 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3601 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3661 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3721 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     3781 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     3841 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     3901 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     3961 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     4021 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     4081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     4141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     4201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     4261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     4321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     4381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     4441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     4501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     4561 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
     4621 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagcttg ccgccaccat
     4681 gagcaagggc gaggaactgt tcactggcgt ggtcccaatt ctcgtggaac tggatggcga
     4741 tgtgaatggg cacaaatttt ctgtcagcgg agagggtgaa ggtgatgcca catacggaaa
     4801 gctcaccctg aaattcatct gcaccactgg aaagctccct gtgccatggc caacactggt
     4861 cactaccctg acctatggcg tgcagtgctt ttccagatac ccagaccata tgaagcagca
     4921 tgactttttc aagagcgcca tgcccgaggg ctatgtgcag gagagaacca tctttttcaa
     4981 agatgacggg aactacaaga cccgcgctga agtcaagttc gaaggtgaca ccctggtgaa
     5041 tagaatcgag ctgaagggca ttgactttaa ggaggatgga aacattctcg gccacaagct
     5101 ggaatacaac tataactccc acaatgtgta catcatggcc gacaagcaaa agaatggcat
     5161 caaggtcaac ttcaagatca gacacaacat tgaggatgga tccgtgcagc tggccgacca
     5221 ttatcaacag aacactccaa tcggcgacgg ccctgtgctc ctcccagaca accattacct
     5281 gtccacccag tctgccctgt ctaaagatcc caacgaaaag agagaccaca tggtcctgct
     5341 ggagtttgtg accgctgctg ggatcacaca tggcatggac gagctgtaca aggctatcga
     5401 tgctctcgac gctaggcccg ggcccgctag cggccttaag cttccgaaga agaagcgaaa
     5461 ggtatctaga gggcccgggc ctagaatgcc ttctgaacaa tggaaaggca ttattgccgt
     5521 aagccgtggc ggtctgatac cggtgggtga agaccagaaa cagcacctcg aactgagccg
     5581 cgatattgcc cagcgtttca acgcgctgta tggcgagatc gatcccgtcg ttttacaacg
     5641 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     5701 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     5761 cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg
     5821 gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca
     5881 cggttacgat gcgcccatct acaccaacgt gacctatccc attacggtca atccgccgtt
     5941 tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg
     6001 gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg
     6061 gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct
     6121 gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gctggagtga
     6181 cggcagttat ctggaagatc aggatgtgtg gcggatgagc ggcattttcc gtgacgtctc
     6241 gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga
     6301 tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta
     6361 cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc
     6421 tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct
     6481 gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt
     6541 tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg
     6601 cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg
     6661 cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt
     6721 gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc
     6781 gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc
     6841 caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct
     6901 accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt
     6961 gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg
     7021 ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac
     7081 cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc
     7141 ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct
     7201 gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg
     7261 gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca
     7321 gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg
     7381 cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc
     7441 gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg
     7501 gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca
     7561 ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc
     7621 tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca
     7681 actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca
     7741 catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc
     7801 gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa
     7861 taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa
     7921 aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat
     7981 tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc
     8041 gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc
     8101 ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg
     8161 gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc
     8221 gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga
     8281 gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc
     8341 ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag
     8401 cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg
     8461 cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg
     8521 ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat
     8581 tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttccagctga gcgccggtcg
     8641 ctaccattac cagttggtct ggtgtcaaaa ataactcgag actagagggc cctattctat
     8701 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     8761 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     8821 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     8881 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     8941 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     9001 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     9061 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     9121 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     9181 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     9241 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     9301 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     9361 ttgattta
//
