LOCUS       sequence-35292-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3357 (=HL 716 + pHL 1191)
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             132..143
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    313..329
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(381..467)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     620..637
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(723..743)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggcggccctc gccctcgccc tcgatcttcg aactcgtggc cgttcacgga gccctccatg
       61 tgcaccttga agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc
      121 accatcgtac gtttctcctc tttaataagc tttgagcctg gcttatcgcc gggctttttt
      181 atggcaaaaa aaagcggatc ctggagatcc gcaaaagttc acgttggctt tagttattcg
      241 agttgagaaa ctctcgaaac gggcagtgac ttcaagggtt aaaagaggtg ccgctccgtt
      301 tccgggagta tcttgttatc cgctcacaat gtcaatgtta tccgctcaca tttatggatc
      361 ccctaggtct agggcggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
      421 ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctggcagtt
      481 ccctactctc gcatggggag accccacact accatcggcg ctacggcgtt tcacttctga
      541 gttcggcatg gggtcaggtg ggaccaccgc gctactgccg ccaggcaaat tctgttttat
      601 cagaccgctt ctgcgttctg atttaatctg tatcaggctg aaaatcttct ctcatccgcc
      661 aaaacagcca agctagcttt tatttgtaga gctcatccat gccatgtgta atcccagcag
      721 ctgttacaaa ctcaagaagg accatgtggt ctctcttttc gttgggatct ttcgaaaggg
      781 cagattgtgt ggacaggtaa tggttgtctg gtaaaaggac agggccatcg ccaattggag
      841 tattttgttg ataatggtct gctagttgaa cgcttccatc ttcaatgttg tgtctaattt
      901 tgaagttaac tttga
//
