LOCUS       sequence-352931-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent 
            minicircle).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Yarnall MTN, Ioannidi EI, Schmitt-Ulms C, Krajeski RN, Lim J, 
            Villiger L, Zhou W, Jiang K, Garushyants SK, Roberts N, Zhang L, 
            Vakulskas CA, Walker JA 2nd, Kadina AP, Zepeda AE, Holden K, Ma H, 
            Xie J, Gao G, Foquet L, Bial G, Donnelly SK, Miyata Y, Radiloff DR, 
            Henderson JM, Ujita A, Abudayyeh OO, Gootenberg JS
  TITLE     Drag-and-drop genome insertion of large sequences without 
            double-strand DNA cleavage using CRISPR-directed integrases.
  JOURNAL   Nat Biotechnol. 2022 Nov 24. doi: 10.1038/s41587-022-01527-4.
  PUBMED    36424489
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Nov 24. doi: 10.1038/s41587-022-01527-4."
COMMENT     Sequence Label: pDY0423 Cargo EGFP with AttP Bxb1 site for in-...
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(469..486)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    507..540
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
ORIGIN
        1 tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca
       61 aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc
      121 gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg
      181 aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgattctt
      241 ctctcatccg ccaaaacagc caagctggag accgtttgac attaccctgt tatccctaga
      301 tacattaccc tgttatccca gatgacatac cctgttatcc ctagatgaca ttaccctgtt
      361 atcccagatg acattaccct gttatcccta gatacattac cctgttatcc cagatgacat
      421 accctgttat ccctagatga cattaccctg ttatcccaga taaactcaat gatgatgatg
      481 atgatggtcg agactcagcg gccgcggtgc cagggcgtgc ccttgggctc cccgggcgcg
      541 gccgccacca tgcccgccat gaagatcgag tgccgcatca ccggcaccct gaacggcgtg
      601 gagttcgagc tggtgggcgg cggagagggc acccccgagc agggccgcat gaccaacaag
      661 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc
      721 tacggcttct accacttcgg cac
//
