LOCUS       sequence-352932-         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cargo EGFP with AttP Bxb1 site for in-frame fusion (Parent 
            minicircle).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Yarnall MTN, Ioannidi EI, Schmitt-Ulms C, Krajeski RN, Lim J, 
            Villiger L, Zhou W, Jiang K, Garushyants SK, Roberts N, Zhang L, 
            Vakulskas CA, Walker JA 2nd, Kadina AP, Zepeda AE, Holden K, Ma H, 
            Xie J, Gao G, Foquet L, Bial G, Donnelly SK, Miyata Y, Radiloff DR, 
            Henderson JM, Ujita A, Abudayyeh OO, Gootenberg JS
  TITLE     Drag-and-drop genome insertion of large sequences without 
            double-strand DNA cleavage using CRISPR-directed integrases.
  JOURNAL   Nat Biotechnol. 2022 Nov 24. doi: 10.1038/s41587-022-01527-4.
  PUBMED    36424489
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Nov 24. doi: 10.1038/s41587-022-01527-4."
COMMENT     Sequence Label: pDY0423 Cargo EGFP with AttP Bxb1 site for in-...
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..106
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     CDS             127..144
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cagctcgaac tccacgccgt tcagggtgcc ggtgatgcgg cactcgatct tcatggcggg
       61 catggtggcg gccgcgcccg gggagcccaa gggcacgccc tggcaccgcg gccgctgagt
      121 ctcgaccatc atcatcatca tcattgagtt tatctgggat aacagggtaa tgtcatctag
      181 ggataacagg gtatgtcatc tgggataaca gggtaatgta tctagggata acagggtaat
      241 gtcatctggg ataacagggt aatgtcatct agggataaca gggtatgtca tctgggataa
      301 cagggtaatg tatctaggga taacagggta atgtcaaacg gtctccagct tggctgtttt
      361 ggcggatgag agaagaatcg ccagcgtcgc acagcatgtt tgcttgtcgc cgtcgcgtct
      421 gtcacatctt ttccgccagc agttagggat tagcgtctta agctggcgcg aggaccaacg
      481 tatcagccag gcgaagctgc ttttgagcac cacccggatg cctatcgcca ccgtcggtcg
      541 caatgttggt tttgacgatc aactctattt ctcgcgggta tttaaaaaat gcaccggggc
      601 cagcccgagc gagttccgtg ccggttgtga agaaaaagtg aatgatgtag ccgtcaagtt
      661 gtcataattg gtaacgaatc agacaattga cggcttgacg gagtagcata gggnttgcag
      721 aatccctgct tcgtccattt gacagg
//
