LOCUS       sequence-352936-       16425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression vector for Saccharomyces cerevisiae 
            comprising the polyketide synthase fsr1 from Fusarium vanettenii and
            the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16425)
  AUTHORS   Bejenari M, Nielsen L, Spedtsberg EML, Nielsen MR, Pedersen TB, 
            Sorensen JL
  TITLE     Yeast recombinational cloning for heterologous biosynthesis of 
            polyketides: a molecular microbiology laboratory module for 
            undergraduate students.
  JOURNAL   J Microbiol Biol Educ. 2023 Nov 27;24(3):e00242-22. doi: 
            10.1128/jmbe.00242-22. eCollection 2023 Dec.
  PUBMED    38108002
REFERENCE   2  (bases 1 to 16425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/jmbe.00242-22"; journalName: "J Microbiol Biol Educ"; date:
            "2023-11-27- 27"; volume: "24"; issue: "3"; pages: "e00242-22"
COMMENT     Sequence Label: pCOA-Leu-FvPPT1-fsr1
FEATURES             Location/Qualifiers
     source          1..16425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     396..415
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(909..2003)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(2004..2411)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(2712..3167)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2849..2870)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3061..3080
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(3246..3411)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     3332..3356
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     11865..11884
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11865..11883
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      12832..13021
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(13093..13110)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13264..13852)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13344..13363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14023..14814,14815..14883))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14646..14665
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14884..14988)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      15015..16357
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(15978..16025)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     16398..16416
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gccaattatg ttatgtctga
       61 taaaaggatg taacataggc aagctgctcg tgagtgttga gtacgaacct tagatccaaa
      121 tcacccgcac cctacggata tacttgcttg aatatacagt agtatttgta ataagttcat
      181 aattaatatc ttaccaaatt aggtaatctg caaaagttca gactgtgaaa tgtaacattt
      241 tatatatcaa gctctattta atgcctcaca gtagttaaca taaagacagc tcccggagac
      301 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
      361 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
      421 agtgcaccat atcgactacg tcgttaaggc cgtttctgac agagtaaaat tcttgaggga
      481 actttcacca ttatgggaaa tggttcaaga aggtattgac ttaaactcca tcaaatggtc
      541 aggtcattga gtgtttttta tttgttgtat tttttttttt ttagagaaaa tcctccaata
      601 tataaattag gaatcatagt ttcatgattt tctgttacac ctaacttttt gtgtggtgcc
      661 ctcctccttg tcaatattaa tgttaaagtg caattctttt tccttatcac gttgagccat
      721 tagtatcaat ttgcttacct gtattccttt acatcctcct ttttctcctt cttgataaat
      781 gtatgtagat tgcgtatata gtttcgtcta ccctatgaac atattccatt ttgtaatttc
      841 gtgtcgtttc tattatgaat ttcatttata aagtttatgt acaaatatca taaaaaaaga
      901 gaatcttttt aagcaaggat tttcttaact tcttcggcga cagcatcacc gacttcggtg
      961 gtactgttgg aaccacctaa atcaccagtt ctgatacctg catccaaaac ctttttaact
     1021 gcatcttcaa tggccttacc ttcttcaggc aagttcaatg acaatttcaa catcattgca
     1081 gcagacaaga tagtggcgat agggttgacc ttattctttg gcaaatctgg agcagaaccg
     1141 tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg gcaaagaggc caaggacgca
     1201 gatggcaaca aacccaagga acctgggata acggaggctt catcggagat gatatcacca
     1261 aacatgttgc tggtgattat aataccattt aggtgggttg ggttcttaac taggatcatg
     1321 gcggcagaat caatcaattg atgttgaacc ttcaatgtag ggaattcgtt cttgatggtt
     1381 tcctccacag tttttctcca taatcttgaa gaggccaaaa cattagcttt atccaaggac
     1441 caaataggca atggtggctc atgttgtagg gccatgaaag cggccattct tgtgattctt
     1501 tgcacttctg gaacggtgta ttgttcacta tcccaagcga caccatcacc atcgtcttcc
     1561 tttctcttac caaagtaaat acctcccact aattctctga caacaacgaa gtcagtacct
     1621 ttagcaaatt gtggcttgat tggagataag tctaaaagag agtcggatgc aaagttacat
     1681 ggtcttaagt tggcgtacaa ttgaagttct ttacggattt ttagtaaacc ttgttcaggt
     1741 ctaacactac cggtacccca tttaggacca cccacagcac ctaacaaaac ggcatcagcc
     1801 ttcttggagg cttccagcgc ctcatctgga agtggaacac ctgtagcatc gatagcagca
     1861 ccaccaatta aatgattttc gaaatcgaac ttgacattgg aacgaacatc agaaatagct
     1921 ttaagaacct taatggcttc ggctgtgatt tcttgaccaa cgtggtcacc tggcaaaacg
     1981 acgatcttct taggggcaga cattagaatg gtatatcctt gaaatatata tatatatatt
     2041 gctgaaatgt aaaaggtaag aaaagttaga aagtaagacg attgctaacc acctattgga
     2101 aaaaacaata ggtccttaaa taatattgtc aacttcaagt attgtgatgc aagcatttag
     2161 tcatgaacgc ttctctattc tatatgaaaa gccggttccg gcgctctcac ctttcctttt
     2221 tctcccaatt tttcagttga aaaaggtata tgcgtcaggc gacctctgaa attaacaaaa
     2281 aatttccagt catcgaattt gattctgtgc gatagcgccc ctgtgtgttc tcgttatgtt
     2341 gaggaaaaaa ataatggttg ctaagagatt cgaactcttg catcttacga tacctgagta
     2401 ttcccacagt taactgcggt caagatattt cttgaatcag gcgccttaga ccgctcggcc
     2461 aaacaaccaa ttacttgttg agaaatagag tataattatc ctataaatat aacgtttttg
     2521 aacacacatg aacaaggaag tacaggacaa ttgattttga agagaatgtg gattttgatg
     2581 taattgttgg gattccattt ttaataaggc aataatatta ggtatgtaga tatactagaa
     2641 gttctcctcg accggtcgat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     2701 accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     2761 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2821 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2881 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2941 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     3001 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     3061 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     3121 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
     3181 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     3241 aattggagcg acctcatgct atacctgaga aagcaacctg acctacagga aagagttact
     3301 caagaataag aattttcgtt ttaaaaccta agagtcactt taaaatttgt atacacttat
     3361 tttttttata acttatttaa taataaaaat cataaatcat aagaaattcg cttatttaga
     3421 agtgtcaaca acgtatctac caacgatttg acccttttcc atcttttcgt aaatttctgg
     3481 caaggtagac aagccgacaa ccttgattgg agacttgacc aaaccttcaa actcttggac
     3541 cccacaatct caatctttcg gcttcaaaac ctgccttatc ttctgcagtt gttttcaaga
     3601 aaccaatttc tttccagata tggatgtact ttctagtaac aacttcagaa actggaccaa
     3661 cagctctcaa tgacttagaa tgttccaaag tatttttaac atccaaaact aaaccaccac
     3721 aagccattct caaatatgtt gattccaaga aatcaaccaa caaagctgct ggattatctt
     3781 gttgtggagc ttctctaacc aactttaacc agtctttaaa aggaaccaaa tttgggattc
     3841 tcaaagtatc agacaagata gcgttaacat ctctccattg ttgaccaact ggattatcaa
     3901 tatggtaaaa tggatgtggt gttctatctg acaaaaccaa atcagacaaa gtatcagcga
     3961 tatcgttaac tggtgtccaa ttcaatgcac catcaacatc tggcaaagca ttcaaagttt
     4021 gtgaagattt aatcaagaaa ccgaaatgtt ccattggatt ccaataacct gatgttttag
     4081 aacctgcaat ttgacccaat ctaacaacca tggctctgaa tctgtttggg tacttatgca
     4141 aagtttcatc caacattctt tcacaacccc attttgcttc agcataaccg tttgacaaca
     4201 aagagtcgac tgtagcagat ttttctggaa caacagtctt ttctgtttga ccagctgcta
     4261 aaccgtattg accaacaaca ccaattgaag aaaccatttg gaatgaaaat ttaaaagatt
     4321 ctggtcttaa agaagctgct tcattaccaa aatcaaccaa atttctaaaa acttggaatt
     4381 gtgattcgaa accagacaat ggtctttttg ctgacattgg ccaagcatta tgaatcaaat
     4441 gagtaacaga accaaccaaa ttatcatagt cacccttatc caaacccaaa tgtggctttg
     4501 aagaatctgt ttggaagatt ctcaacttat gcttcaaatt ttctgggaat ctgataccct
     4561 tttctctcat agccttgtat tgtctagttt ctggaccttc tctattatct ctattcaaac
     4621 aaataactgt cttaacatct tccaattgag cgattgagta aaccaaatga gaacctaaac
     4681 caccagttgc acctgtaatc aaaacaacct tttcgttgtt tgctggcaaa acagctgatg
     4741 gtgaagaagt tggtgcagaa aaaccagctg tgtacttttg aaccaattca tcaactgcct
     4801 tttgtctagc atccaaatct gaagtaccag aatgagcgtt gttaacaaca actgtctttg
     4861 atggagatgc ttgaacagct tctggaactg gtaaagctgc tggcttatta actggaacag
     4921 atggaactgg tggagtacca cccatttctc tcatcaacat ttctttcaag tcaccaacag
     4981 tttgacaatc caaaaataat gtttcatgac caacatctaa accgaaagca tcttggaatc
     5041 tagaagtaat aaccaatgac aacaaagaat caacaccaca atcagcgaag tttgtatcat
     5101 catccaattc ttctcttgca acacctgatt cttcagagat gatatccaaa gctctcaaga
     5161 aatcaacttc gaatgggttt gcgaaatctt catgagcatc tggagtaact gcacctgtaa
     5221 tcaaacctga tggtggagat tctaaacctg aatcagatgc atttgaagat gaagtagcag
     5281 aaccatcatc atctggaccc atcaagaaaa tcttcaaatc ttgaactgtt ggatgatcga
     5341 aaaacaaagc gttgaaatcc aaatcgatat ccaattcttc tctgaatctt gctgagatag
     5401 tcaaacccaa caatgaatcg ataccaacat cagagaagtt agtaccatct gtcaaatcag
     5461 caacatcaat accagattct tcagcaatga tagtcaatgc tgttgagatt ctagatggtt
     5521 gaaccttttc aactggcaat ggcaactttt ggataccaga tgaaccattt gctggagctg
     5581 agatagagat agttggtgtc ttattttgag ctggtctttg tggttgagtc tttttacctg
     5641 attccaaaga caaaataact ttcaaaactc ttcttggaac accttgaatt gcaatttgac
     5701 caaagaaagc aacaactctg tcaccatcaa aaataacaac gtcaccatgc cacaatttat
     5761 cttcacctgg cctcatttga gtgtaagttg tgtatgtctt atcgaattgc aatggttcga
     5821 acatttgtaa agaaccccaa ccatggttca taaaaacttc aacatccaaa tcagtatcat
     5881 cgttacagtt catagcgaaa ccacaacctt gagtcaatga atcgatgatt gctggatgtg
     5941 tatggaagtt accatctctc ttaactgaac cgaaagacaa ttgagatgaa gcttcgtatg
     6001 ttggagaatt caagatgatt tcatcgattg ctctgtaatc atcatggaat ctagctaatg
     6061 gtctaattgc tctaaaaacc atagctctgt tgtatcttgc agtttcacct gtagcaatac
     6121 catttctcaa tgcttgcatc ttttgcttaa cagaaacagc cttatcttgc aaagtctttt
     6181 gcaatgaatg atctctgaat ctaacaacac attcagaatg cttttgcaaa ttacccttat
     6241 tatcgaaagt cataaactta atagttgctg attgagatgc ccaatcagca tccaaatgag
     6301 cttgtaatgg ttgtttagaa ccgtcacctc tcaagatcaa agcctttgag attgtcatat
     6361 cagaaacatc gatcaaattt tctttctttt gtggttggta tctttccaac aaatatctac
     6421 ccaaagttaa tgccatatca gcgtaaacag atggtgtaca taatggaata ccatcaactt
     6481 catgaccttg aaccaatggt gacatatcct ttctagagat atcagcttca acgatcatat
     6541 gagttttctt agagtcacca gattcttcaa caactgaatg gatagttgta gattccaatc
     6601 ttggtgcgtt attaataacg attggagcgt caccttttct caaagaccaa tcgttaacgt
     6661 attggatcca gtaatccttc aaatcccatg agtaagctgg taatggaaca acagaatgaa
     6721 aaccaccaaa atctctttgg tattctgccc atgaaatatt tgcaccagca tcatacaaaa
     6781 ccttcaaagc tgcacacaaa acatcccatg gttgtcttgc tctttgcaaa gaagcgatac
     6841 aaataacttg ttgacccaaa actgccttaa ccataccagt aatagctgga tgtggaccca
     6901 attccaagat cattgactta tcagtgataa catgatctct gtatgctgta gacaaagcac
     6961 ctaacatgtt aactgattgt ctagaatggt tagccaaata ttctggacca aaagaatcac
     7021 aatgatcaac aactgtacca tccaatggtc ttaaaactgg aactgatggt ttagaaaaag
     7081 taacacctgt agcaatttct tgaaattctg acaaaactgg atccaattga gatgaatgga
     7141 atgcgtatgg aaccttcaac aaagttgact tcatagaagc tgctgtcaac aattccttca
     7201 aatctgcaac ttgttcgtta gtaccagcta aaactgtttc gattggtgag ttgatacatg
     7261 cagtttcgta ttcgttaccc ttcaaaactt cagcgatttc atcttcagaa cctttaacaa
     7321 ccaacattga atgagtagat cttgtacact tttgttccaa caattgagct cttttaccaa
     7381 ccaaaaataa agtatctgta tctgacaaaa cacctgcaac atttaaagct gcgtattcac
     7441 ccaaagaatg accaacaaca gctgctggat agatacccca tgaagcccac aacttggaca
     7501 aagtgatttg caaacaaact gatgccaatt gaactgctgt tggagcaaaa ataccgatat
     7561 cttgttcttc agattgaata actggtaaca ttgatggaaa acccaaagat tgagcgattt
     7621 gatccaattg gatcaattca gatctaacga aagatgattc ttcgaacaat tgtttaccca
     7681 taccagggta ttgagcacct tgaccagtaa aagtaaaaat aacagatggt gcagactttg
     7741 gtctgttaac accagtgttg ttttgcaaag ctgtttcaat tttcttacag atatcttcag
     7801 ttgttggacc attcaacaaa actctatgtt gatgatggat tcttctagca gttgtagtgt
     7861 atgacaattc acccaaagaa acttctggat tttctttcaa gaacttcaac attgatctca
     7921 tgttaccctg caaagctgaa ccagattttg cagtaacagc aattggaaat tgaactctag
     7981 aatcaactgt tgctcttctt tcaacttttg gtggtgcatc ttccaataac aaagctgaat
     8041 taccaccagc tgcagagaag ttgttaacga aaactcttct tggaccatta attctatccc
     8101 atggagttgg cttcaaagcg atatgaacgt ttctttgcaa caaatcagtt ggaaacttat
     8161 gattaatctt tgttttaata ccacaatgtg gaacgatcat attcttttgc atcatgttca
     8221 aaactttaat aacagatgaa gcaccagaag ctgcttcacc atgaccaatg tttgccttag
     8281 ctgaacccaa aaataatgct tgttcatcag atctagcctt tttaccatta accaatggtg
     8341 gtgcaaaagt atctaaaact gaagccattt ctgttgcgtc accagcttga gtacctgtac
     8401 catgcatttc aacgtaagaa acagtatatg gatcaacagc tgcttctgac aagatcttag
     8461 agaaaattgc tctttgagca ccaacatgtg gtctagtaat tgattcagat tctgcagaat
     8521 gatttgtgta agcacccaaa ataacaccca agattggatc gttttctgcg atagcatcat
     8581 ccaatctctt aataatcaat gttgcaacac cttcacctct acaataacca tcagcaccat
     8641 catcgaaagt cttacagtta ccagttcttg acaagaaatg acctctatcc aaaccagaag
     8701 tgaaatctgg gttagtcaaa acgtttgtac cacctgcaat agctgtatca atgtcacctc
     8761 tccataaaga attacatgcc aaatgaatac cagctaaaga tgaagaacat gcagtatcaa
     8821 cagcgtatga tggaccagaa aatttaaaga aataattaat tctacctgga ataaaagctc
     8881 tattaccacc tggaataaag tatgtatcaa taccttgtgc tgagttagtt tccatccaat
     8941 cgttagatgt gacaccatag aaaacaccaa ctctatctgg tctagtagat ggtgttgcat
     9001 ctggaacaat accagcttgt tcaattgctt cataagcagt cattaatgca attctttgag
     9061 ctggatcaat ttgtggagct tctcttggag agatgttgaa aaatcttgca tcgaattcag
     9121 ctggatcatc caaccaacaa ccaaatggag tagctgatgt atttttagtt ttacctgttg
     9181 gatcaacatg tgtttgtgca tcccatctca aagatggaac tggcttatga acatctaaac
     9241 cttggaacaa caaatcccag tatgcttcat tatctttagc acctggaaat ctaccagaca
     9301 ttgcaacgat agccaacttt ggtttcttag aagtagattc aacaccaaca tctggaactg
     9361 attgtctacc ttgaccatgt gtatttggaa ccaaagatct taatggagtt tgcttcaatg
     9421 ctgtatagat caaatgatca gcagttgaac caattggaac aacagaaaat tgttctggat
     9481 taacatcttg caaacaagct tgaatgtcac ctaaaattga agaccaatgc aatggatgaa
     9541 ctaatgcttg agcaactgca tcttctaaca aagtagcgaa tgatctttct tccaacttaa
     9601 cacctgttga agataacaat ggaatttgtt cagacaaagc agtagctgca ttgtatgata
     9661 aaccagaaac aattttctta gcatccattt gagagtacaa atgtggtgca tggtaagcac
     9721 cgaagattgg gattgactta gatgtcaaac ctttaaaaga tggtgaagag atcaattgag
     9781 ctaaagtttt tggtggacca gaaactgtaa taccatttgg agcgtatgct gaaatatatg
     9841 gtctagaagt caatggcaaa ttagattctt cacagaactt ttcaacagct gcagctgatt
     9901 tagaacctgg aactaacaaa gcccatgatt gatccaaatc ttgagattga tcaactcttt
     9961 ttgcaacatc agtaaccaac atgcctgttc taaaagcagc aataactgaa taaactgcca
    10021 ttggaatcaa atcagcagta aaagaagaac aagagattgc agctgcagca aaagcacctg
    10081 tacacaaacc aacaactctt gaagattgtg catcgtatga agagatacca gcttgtgaaa
    10141 taaaagtacc caaatggtac aatgtagtca ttgccatatc caaagcgatg catcttttac
    10201 cagattcatc ccatcttaac aaatcttctt gacaagtgaa tcttggcaaa tcgtttctaa
    10261 ctttaaaagg caatctgtac atttcaactc tgattgcgtt gtaagccttt gtcaagaaat
    10321 cttgcaaaaa caaatggtct ctttgttgga ataatttttg taagtgtggc ttgatgtcga
    10381 aggtttggtc accgaataag tataatttta agttgtctgt catgcccttt agtgagggtt
    10441 gaattcgaat tttgaatgat tcttatactc agaaggaaat gcttaacgat ttcgggtgtg
    10501 agttgacaag gagagagaga aaagaagagg aaaggtaatt cggggacggt ggtcttttat
    10561 acccttggct aaagtcccaa ccacaaagca aaaaaatttt cagtagtcta ttttgcgtcc
    10621 ggcatgggtt acccggatgg ccagacaaag aaactagtac aaagtctgaa caagcgtaga
    10681 ttccagactg cagtacccta cgcccttaac ggcaagtgtg ggaaccgggg gaggtttgat
    10741 atgtggggtg aagggggctc tcgccggggt tgggcccgct actgggtcaa tttggggtca
    10801 attggggcaa ttggggctgt tttttgggac acaaatacgc cgccaacccg gtctctgagt
    10861 ttggcgcccg ttttttcgag ccccacacgt ttcggtgagt atgagcggcg gcagattcga
    10921 gcgtttccgg tttccgcggc gggacgagag cccatgatgg gggctcccac caccagcaat
    10981 cagggccctg attacacacc cacctgtaat gtcatgctgt tcatcgtggt taatgctgct
    11041 gtgtgctgtg tgtgtgtgtt gtttggcgct cattgttgcg ttatgcagcg tacaccacaa
    11101 tattggaagc ttattagcct ttctattttt tcgtttgcaa ggcttaacaa cattgctgtg
    11161 gagagggatg gggatatgga ggccgctgga gggagtcgga gaggcgtttt ggagcggctt
    11221 ggcctggcgc ccactcgcga aacgcaccta ggaccctttg gcacgccgaa atgtgccact
    11281 tttcagtcta gtaacgcctt acctacgtca ttccatgcat gcatgtttgc gccttttttc
    11341 ccttgccctt gatcgccaca cagtacagtg cactgtacag tggaggtttt gggggggtct
    11401 tagatgggag ctaaaagcgg cctagcggta cactagtggg attgtatgga gtggcatgga
    11461 gcctgggtgg agcctgacag gacgcacgac cggctagccc gtgacagacg atgggtggct
    11521 cctgttgtcc accgcgtaca aatgtttggg ccaaagtctt gtcagccttg cttgcgaacc
    11581 taattcccaa ttttgtcact tcgcaccccc attgatcgag ccctaacccc tgcccatcag
    11641 gcaatccaat taagctcgca ttgtctgcct tgtttagttt ggctcctgcc cgtttcggcg
    11701 tccacttgca caaacacaaa caagcattat atataaggct cgtctctccc tcccaaccac
    11761 actcactttt ttgcccgtct tcccttgcta acacaaaagt caagaacaca aacaaccacc
    11821 ccaaccccct tacacacaag acatatctac agcacccgga tccgtaatac gactcactat
    11881 agggcccggg cgtcgacatg gaacagaagt tgatttccga agaagaccat gtcctcagca
    11941 caatcatcac caacagtaat acaatgggta atagatacaa gacctttatg gccttccgtt
    12001 ttgaagacta aagatttgac ttctgctgca tcaagagctt tgtctttgtt gacagaagaa
    12061 gaacaatctt cagttttgag atactaccat gttagagatg caaaattggc tttagcatct
    12121 gctttgttga agagatacgc tatctcaaga ttttgtcatg ttccatggtt tcaagctaaa
    12181 actacaagag atgcaagaac taaaccagtt tttgttttgc catctggtga cgaaccattg
    12241 attttcaatg tttcacatca agcaggtttg gctgttttgt tagcagttca tgatccacca
    12301 aaaggtttag ctattggtgt tgatgttgtt tgtccatctg aaagaagaga tagagatttg
    12361 tcttcattag aagaagatgg ttgggcatca ttcgttgata tccatgcaga tgtttttggt
    12421 gctggtgaag tttctgcatt gaaatcaatg aatccagttc caactgctca agatagagat
    12481 agagcattga gatacttcta cgctttgtgg tgtttgagag aagcttacgt taagatgaca
    12541 ggtgacgcat tgttagcttc ttggttgaag gatttggaaa tgcataactt cgcaccacca
    12601 gaagatatga aagaagctca agaagttaga ttgagaggta aaaaggttga aggtgttgat
    12661 gttagattgg ttccattgtt ggaagaatac atgatctcta cagctatcag aaacggtgac
    12721 aacggtgaat caattaaatt aggtgaattt caaaacttaa acatggaaga agtattggaa
    12781 ttcggtgaaa aggctcctaa atcataaagc ttggtaccgc ggctagctaa gatccgctct
    12841 aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt tttttatagt
    12901 tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc tgtacagacg
    12961 cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg gacgctcgaa
    13021 gatccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
    13081 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
    13141 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    13201 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    13261 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    13321 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    13381 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    13441 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    13501 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    13561 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    13621 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    13681 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
    13741 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    13801 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    13861 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    13921 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    13981 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    14041 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    14101 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    14161 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    14221 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    14281 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    14341 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    14401 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    14461 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    14521 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    14581 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    14641 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    14701 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    14761 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    14821 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    14881 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    14941 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    15001 aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga
    15061 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg
    15121 cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa
    15181 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
    15241 caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa
    15301 tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg
    15361 tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa
    15421 gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg
    15481 tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta
    15541 tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat
    15601 tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat
    15661 aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa
    15721 tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag
    15781 atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat
    15841 atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt
    15901 agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg
    15961 gttttcaaaa gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga
    16021 acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac
    16081 agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga
    16141 agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg
    16201 atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc
    16261 ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct
    16321 catccttcaa tgctatcatt tcctttgata ttggatcata ctaagaaacc attattatca
    16381 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
