LOCUS       sequence-35294-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3330 (=HL 770 + pHL 1009)
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             125..136
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    291..307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(359..445)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     598..615
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(701..721)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc atgtgcacct
       61 tgaagcgcat gaactccttg atgatggcca tgttatcctc ctcgcccttg ctcaccatcg
      121 tacgtttctc ctctttaata agcttctgga taaggattat ccaattctaa aaaaaaagcc
      181 cggacgactg ttcgggcttg tctttttata tgttggaaaa tcagtggcaa tgcaatggcc
      241 caacagaaaa taaaatctgt gacaataaag gcatataacc cgggagtatc ttgttatccg
      301 ctcacaatgt caatgttatc cgctcacatt tatggatccc ctaggtctag ggcggcggat
      361 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
      421 tcgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc atggggagac
      481 cccacactac catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg
      541 accaccgcgc tactgccgcc aggcaaattc tgttttatca gaccgcttct gcgttctgat
      601 ttaatctgta tcaggctgaa aatcttctct catccgccaa aacagccaag ctagctttta
      661 tttgtagagc tcatccatgc catgtgtaat cccagcagct gttacaaact caagaaggac
      721 catgtggtct ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg acaggtaatg
      781 gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc
      841 tagttgaacg cttccatctt caatgttgtg tctaattttg aagttaactt tgattccatt
      901 cttttgtttg tctgccatga tgtatacatt
//
