LOCUS       sequence-35295-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3326 (=HL 716 + pHL 1008)
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             124..135
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    281..297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(349..435)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     588..605
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(691..711)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgtgcacctt
       61 gaagcgcatg aactccttga tgatggccat gttatcctcc tcgcccttgc tcaccatcgt
      121 acgtttctcc tctttaataa gcttagcgtc tctgaagtga atcgttgaat gcacaataaa
      181 aaaatcccga ccctgagggg gtcgggatga aacttgctta agcaagaagc acttaaaaaa
      241 ttcgttacac caggaaatct gatgtgttcc cgggagtatc ttgttatccg ctcacaatgt
      301 caatgttatc cgctcacatt tatggatccc ctaggtctag ggcggcggat ttgtcctact
      361 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc
      421 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc atggggagac cccacactac
      481 catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc
      541 tactgccgcc aggcaaattc tgttttatca gaccgcttct gcgttctgat ttaatctgta
      601 tcaggctgaa aatcttctct catccgccaa aacagccaag ctagctttta tttgtagagc
      661 tcatccatgc catgtgtaat cccagcagct gttacaaact caagaaggac catgtggtct
      721 ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg acaggtaatg gttgtctggt
      781 aaaaggacag ggccatcgcc aattggagta ttttgttgat aatggtctgc tagttgaacg
      841 cttccatctt caatgttgtg tctaattttg aagttaactt tgattccatt ctttgtttgt
      901 ctgcc
//
