LOCUS       sequence-35296-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3327 (=HL 716 + pHL 1009)
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             124..135
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    290..306
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(358..444)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     597..614
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(700..720)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cctcgcctcg ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgtgcacctt
       61 gaagcgcatg aactccttga tgatggccat gttatcctcc tcgcccttgc tcaccatcgt
      121 acgtttctcc tctttaataa gcttctggat aaggattatc caattctaaa aaaaaagccc
      181 ggacgactgt tcgggcttgt ctttttatat gttggaaaat cagtggcaat gcaatggccc
      241 aacagaaaat aaaatctgtg acaataaagg catataaccc gggagtatct tgttatccgc
      301 tcacaatgtc aatgttatcc gctcacattt atggatcccc taggtctagg gcggcggatt
      361 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt
      421 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgca tggggagacc
      481 ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg tcaggtggga
      541 ccaccgcgct actgccgcca ggcaaattct gttttatcag accgcttctg cgttctgatt
      601 taatctgtat caggctgaaa atcttctctc atccgccaaa acagccaagc tagcttttat
      661 ttgtagagct catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc
      721 atgtggtctc tcttttcgtt gggatctttc gaaagggcag attgtgtgga caggtaatgg
      781 ttgtctggta aaaggacagg gccatcgcca attggagtat tttgttgata atggtctgct
      841 agttgaacgc ttccatc
//
