LOCUS       sequence-352980-        9607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the polyketide synthase Fsr1 from Fusarium vanettenii codon 
            optimized for expression in Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9607)
  AUTHORS   Bejenari M, Nielsen L, Spedtsberg EML, Nielsen MR, Pedersen TB, 
            Sorensen JL
  TITLE     Yeast recombinational cloning for heterologous biosynthesis of 
            polyketides: a molecular microbiology laboratory module for 
            undergraduate students.
  JOURNAL   J Microbiol Biol Educ. 2023 Nov 27;24(3):e00242-22. doi: 
            10.1128/jmbe.00242-22. eCollection 2023 Dec.
  PUBMED    38108002
REFERENCE   2  (bases 1 to 9607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/jmbe.00242-22"; journalName: "J Microbiol Biol Educ"; date:
            "2023-11-27- 27"; volume: "24"; issue: "3"; pages: "e00242-22"
COMMENT     Sequence Label: pUC57-fsr1
FEATURES             Location/Qualifiers
     source          1..9607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7386..7402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7386..7402)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7399..7421)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7410..7426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7434..7440,7441..7458,7459..7464))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7479..7500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7617..7634)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7788..8376)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7868..7887)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8547..9338,9339..9407))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9170..9189
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9408..9512)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9580..9598
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tatgacagac aacttaaaat tatacttatt cggtgaccaa accttcgaca
      481 tcaagccaca cttacaaaaa ttattccaac aaagagacca tttgtttttg caagatttct
      541 tgacaaaggc ttacaacgca atcagagttg aaatgtacag attgcctttt aaagttagaa
      601 acgatttgcc aagattcact tgtcaagaag atttgttaag atgggatgaa tctggtaaaa
      661 gatgcatcgc tttggatatg gcaatgacta cattgtacca tttgggtact tttatttcac
      721 aagctggtat ctcttcatac gatgcacaat cttcaagagt tgttggtttg tgtacaggtg
      781 cttttgctgc agctgcaatc tcttgttctt cttttactgc tgatttgatt ccaatggcag
      841 tttattcagt tattgctgct tttagaacag gcatgttggt tactgatgtt gcaaaaagag
      901 ttgatcaatc tcaagatttg gatcaatcat gggctttgtt agttccaggt tctaaatcag
      961 ctgcagctgt tgaaaagttc tgtgaagaat ctaatttgcc attgacttct agaccatata
     1021 tttcagcata cgctccaaat ggtattacag tttctggtcc accaaaaact ttagctcaat
     1081 tgatctcttc accatctttt aaaggtttga catctaagtc aatcccaatc ttcggtgctt
     1141 accatgcacc acatttgtac tctcaaatgg atgctaagaa aattgtttct ggtttatcat
     1201 acaatgcagc tactgctttg tctgaacaaa ttccattgtt atcttcaaca ggtgttaagt
     1261 tggaagaaag atcattcgct actttgttag aagatgcagt tgctcaagca ttagttcatc
     1321 cattgcattg gtcttcaatt ttaggtgaca ttcaagcttg tttgcaagat gttaatccag
     1381 aacaattttc tgttgttcca attggttcaa ctgctgatca tttgatctat acagcattga
     1441 agcaaactcc attaagatct ttggttccaa atacacatgg tcaaggtaga caatcagttc
     1501 cagatgttgg tgttgaatct acttctaaga aaccaaagtt ggctatcgtt gcaatgtctg
     1561 gtagatttcc aggtgctaaa gataatgaag catactggga tttgttgttc caaggtttag
     1621 atgttcataa gccagttcca tctttgagat gggatgcaca aacacatgtt gatccaacag
     1681 gtaaaactaa aaatacatca gctactccat ttggttgttg gttggatgat ccagctgaat
     1741 tcgatgcaag atttttcaac atctctccaa gagaagctcc acaaattgat ccagctcaaa
     1801 gaattgcatt aatgactgct tatgaagcaa ttgaacaagc tggtattgtt ccagatgcaa
     1861 caccatctac tagaccagat agagttggtg ttttctatgg tgtcacatct aacgattgga
     1921 tggaaactaa ctcagcacaa ggtattgata catactttat tccaggtggt aatagagctt
     1981 ttattccagg tagaattaat tatttcttta aattttctgg tccatcatac gctgttgata
     2041 ctgcatgttc ttcatcttta gctggtattc atttggcatg taattcttta tggagaggtg
     2101 acattgatac agctattgca ggtggtacaa acgttttgac taacccagat ttcacttctg
     2161 gtttggatag aggtcatttc ttgtcaagaa ctggtaactg taagactttc gatgatggtg
     2221 ctgatggtta ttgtagaggt gaaggtgttg caacattgat tattaagaga ttggatgatg
     2281 ctatcgcaga aaacgatcca atcttgggtg ttattttggg tgcttacaca aatcattctg
     2341 cagaatctga atcaattact agaccacatg ttggtgctca aagagcaatt ttctctaaga
     2401 tcttgtcaga agcagctgtt gatccatata ctgtttctta cgttgaaatg catggtacag
     2461 gtactcaagc tggtgacgca acagaaatgg cttcagtttt agatactttt gcaccaccat
     2521 tggttaatgg taaaaaggct agatctgatg aacaagcatt atttttgggt tcagctaagg
     2581 caaacattgg tcatggtgaa gcagcttctg gtgcttcatc tgttattaaa gttttgaaca
     2641 tgatgcaaaa gaatatgatc gttccacatt gtggtattaa aacaaagatt aatcataagt
     2701 ttccaactga tttgttgcaa agaaacgttc atatcgcttt gaagccaact ccatgggata
     2761 gaattaatgg tccaagaaga gttttcgtta acaacttctc tgcagctggt ggtaattcag
     2821 ctttgttatt ggaagatgca ccaccaaaag ttgaaagaag agcaacagtt gattctagag
     2881 ttcaatttcc aattgctgtt actgcaaaat ctggttcagc tttgcagggt aacatgagat
     2941 caatgttgaa gttcttgaaa gaaaatccag aagtttcttt gggtgaattg tcatacacta
     3001 caactgctag aagaatccat catcaacata gagttttgtt gaatggtcca acaactgaag
     3061 atatctgtaa gaaaattgaa acagctttgc aaaacaacac tggtgttaac agaccaaagt
     3121 ctgcaccatc tgttattttt acttttactg gtcaaggtgc tcaataccct ggtatgggta
     3181 aacaattgtt cgaagaatca tctttcgtta gatctgaatt gatccaattg gatcaaatcg
     3241 ctcaatcttt gggttttcca tcaatgttac cagttattca atctgaagaa caagatatcg
     3301 gtatttttgc tccaacagca gttcaattgg catcagtttg tttgcaaatc actttgtcca
     3361 agttgtgggc ttcatggggt atctatccag cagctgttgt tggtcattct ttgggtgaat
     3421 acgcagcttt aaatgttgca ggtgttttgt cagatacaga tactttattt ttggttggta
     3481 aaagagctca attgttggaa caaaagtgta caagatctac tcattcaatg ttggttgtta
     3541 aaggttctga agatgaaatc gctgaagttt tgaagggtaa cgaatacgaa actgcatgta
     3601 tcaactcacc aatcgaaaca gttttagctg gtactaacga acaagttgca gatttgaagg
     3661 aattgttgac agcagcttct atgaagtcaa ctttgttgaa ggttccatac gcattccatt
     3721 catctcaatt ggatccagtt ttgtcagaat ttcaagaaat tgctacaggt gttacttttt
     3781 ctaaaccatc agttccagtt ttaagaccat tggatggtac agttgttgat cattgtgatt
     3841 cttttggtcc agaatatttg gctaaccatt ctagacaatc agttaacatg ttaggtgctt
     3901 tgtctacagc atacagagat catgttatca ctgataagtc aatgatcttg gaattgggtc
     3961 cacatccagc tattactggt atggttaagg cagttttggg tcaacaagtt atttgtatcg
     4021 cttctttgca aagagcaaga caaccatggg atgttttgtg tgcagctttg aaggttttgt
     4081 atgatgctgg tgcaaatatt tcatgggcag aataccaaag agattttggt ggttttcatt
     4141 ctgttgttcc attaccagct tactcatggg atttgaagga ttactggatc caatacgtta
     4201 acgattggtc tttgagaaaa ggtgacgctc caatcgttat taataacgca ccaagattgg
     4261 aatctacaac tatccattca gttgttgaag aatctggtga ctctaagaaa actcatatga
     4321 tcgttgaagc tgatatctct agaaaggata tgtcaccatt ggttcaaggt catgaagttg
     4381 atggtattcc attatgtaca ccatctgttt acgctgatat ggcattaact ttgggtagat
     4441 atttgttgga aagataccaa ccacaaaaga aagaaaattt gatcgatgtt tctgatatga
     4501 caatctcaaa ggctttgatc ttgagaggtg acggttctaa acaaccatta caagctcatt
     4561 tggatgctga ttgggcatct caatcagcaa ctattaagtt tatgactttc gataataagg
     4621 gtaatttgca aaagcattct gaatgtgttg ttagattcag agatcattca ttgcaaaaga
     4681 ctttgcaaga taaggctgtt tctgttaagc aaaagatgca agcattgaga aatggtattg
     4741 ctacaggtga aactgcaaga tacaacagag ctatggtttt tagagcaatt agaccattag
     4801 ctagattcca tgatgattac agagcaatcg atgaaatcat cttgaattct ccaacatacg
     4861 aagcttcatc tcaattgtct ttcggttcag ttaagagaga tggtaacttc catacacatc
     4921 cagcaatcat cgattcattg actcaaggtt gtggtttcgc tatgaactgt aacgatgata
     4981 ctgatttgga tgttgaagtt tttatgaacc atggttgggg ttctttacaa atgttcgaac
     5041 cattgcaatt cgataagaca tacacaactt acactcaaat gaggccaggt gaagataaat
     5101 tgtggcatgg tgacgttgtt atttttgatg gtgacagagt tgttgctttc tttggtcaaa
     5161 ttgcaattca aggtgttcca agaagagttt tgaaagttat tttgtctttg gaatcaggta
     5221 aaaagactca accacaaaga ccagctcaaa ataagacacc aactatctct atctcagctc
     5281 cagcaaatgg ttcatctggt atccaaaagt tgccattgcc agttgaaaag gttcaaccat
     5341 ctagaatctc aacagcattg actatcattg ctgaagaatc tggtattgat gttgctgatt
     5401 tgacagatgg tactaacttc tctgatgttg gtatcgattc attgttgggt ttgactatct
     5461 cagcaagatt cagagaagaa ttggatatcg atttggattt caacgctttg tttttcgatc
     5521 atccaacagt tcaagatttg aagattttct tgatgggtcc agatgatgat ggttctgcta
     5581 cttcatcttc aaatgcatct gattcaggtt tagaatctcc accatcaggt ttgattacag
     5641 gtgcagttac tccagatgct catgaagatt tcgcaaaccc attcgaagtt gatttcttga
     5701 gagctttgga tatcatctct gaagaatcag gtgttgcaag agaagaattg gatgatgata
     5761 caaacttcgc tgattgtggt gttgattctt tgttgtcatt ggttattact tctagattcc
     5821 aagatgcttt cggtttagat gttggtcatg aaacattatt tttggattgt caaactgttg
     5881 gtgacttgaa agaaatgttg atgagagaaa tgggtggtac tccaccagtt ccatctgttc
     5941 cagttaataa gccagcagct ttaccagttc cagaagctgt tcaagcatct ccatcaaaga
     6001 cagttgttgt taacaacgct cattctggta cttcagattt ggatgctaga caaaaggcag
     6061 ttgatgaatt ggttcaaaag tacacagctg gtttttctgc accaacttct tcaccatcag
     6121 ctgttttgcc agcaaacaac gaaaaggttg ttttgattac aggtgcaact ggtggtttag
     6181 gttctcattt ggtttactca atcgctcaat tggaagatgt taagacagtt atttgtttga
     6241 atagagataa tagagaaggt ccagaaacta gacaatacaa ggctatgaga gaaaagggta
     6301 tcagattccc agaaaatttg aagcataagt tgagaatctt ccaaacagat tcttcaaagc
     6361 cacatttggg tttggataag ggtgactatg ataatttggt tggttctgtt actcatttga
     6421 ttcataatgc ttggccaatg tcagcaaaaa gaccattgtc tggtttcgaa tcacaattcc
     6481 aagtttttag aaatttggtt gattttggta atgaagcagc ttctttaaga ccagaatctt
     6541 ttaaattttc attccaaatg gtttcttcaa ttggtgttgt tggtcaatac ggtttagcag
     6601 ctggtcaaac agaaaagact gttgttccag aaaaatctgc tacagtcgac tctttgttgt
     6661 caaacggtta tgctgaagca aaatggggtt gtgaaagaat gttggatgaa actttgcata
     6721 agtacccaaa cagattcaga gccatggttg ttagattggg tcaaattgca ggttctaaaa
     6781 catcaggtta ttggaatcca atggaacatt tcggtttctt gattaaatct tcacaaactt
     6841 tgaatgcttt gccagatgtt gatggtgcat tgaattggac accagttaac gatatcgctg
     6901 atactttgtc tgatttggtt ttgtcagata gaacaccaca tccattttac catattgata
     6961 atccagttgg tcaacaatgg agagatgtta acgctatctt gtctgatact ttgagaatcc
     7021 caaatttggt tccttttaaa gactggttaa agttggttag agaagctcca caacaagata
     7081 atccagcagc tttgttggtt gatttcttgg aatcaacata tttgagaatg gcttgtggtg
     7141 gtttagtttt ggatgttaaa aatactttgg aacattctaa gtcattgaga gctgttggtc
     7201 cagtttctga agttgttact agaaagtaca tccatatctg gaaagaaatt ggtttcttga
     7261 aaacaactgc agaagataag gcaggttttg aagccgaaag attgagattg tggggtccaa
     7321 gagtttgaat cggatcccgg gcccgtcgac tgcagaggcc tgcatgcaag cttggcgtaa
     7381 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7441 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7501 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7561 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7621 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7681 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7741 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7801 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7861 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7921 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7981 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8041 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8101 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8161 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8221 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8281 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8341 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8401 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8461 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8521 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8581 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8641 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8701 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8761 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8821 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8881 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8941 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9001 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9061 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9121 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9181 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9241 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9301 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9361 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9421 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9481 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9541 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     9601 tttcgtc
//
