LOCUS       sequence-352989-       11845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0335
FEATURES             Location/Qualifiers
     source          1..11845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    4783..5371
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4836..4856)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5254..5265)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5443..5676
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5754..5875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5791..5810)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5845..5864
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5915..6050
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6070..6089)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6071..6089)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6099..6116)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6099..6115)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6108..6130)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6257..6712
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6344..6363)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6554..6575
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6738..6842
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6843..6911,6912..7703)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7061..7080)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7874..8462
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8363..8382
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8616..8633
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8750..8771
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8786..8791,8792..8809,8810..8816)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8824..8840
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8829..8851
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8848..8864
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8848..8864
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8883..8903
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8885..8903
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8931..9157
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9158..9338
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9385..9510
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10003..10236
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10421..10465
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10696..10906
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10718..10914
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10765..10900
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10946..11548
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10946..10965)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11442..11462
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11591..11708
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgcc
     1201 aggaacccct ggaagcctag aaatgggact gttgacattt agggatgtgg ccatagaatt
     1261 ctctccggag gagtggcaat gtctggacac tgcacagcag aatttatata ggaatgtgat
     1321 gttagagaac tacagaaacc tggccttcct gggtatatgt cctcattttc ctcaagactt
     1381 ttggccagag cagagcatgg aagattcttt tcaaaaagta ttactgagaa aatatgaaaa
     1441 atgtggacat gagaatttac agttaagaaa aggttgtaaa agtgtggatg agtgtaaggt
     1501 gcacaaagaa ggttataata aacttaacca gtgtctcaca actgcccaga gcaaagtatt
     1561 tcaatgtggg aaatatttga aagtcttcta taaattttta aattcaaaca gacatacgat
     1621 aagacatact ggaaagaaat gcttcaaatg taaaaaatgt gtcaagtcat tttgcatccg
     1681 tttacacaaa acccaacata aatgcgttta tattacagag aagtcctgta aatgtaaaga
     1741 atgtgaaaaa acctttcatt ggtcctcaac ccttactaat cataaggaaa ttcatactga
     1801 agataaaccc tacaaatgtg aagaatgtgg caaagctttt aagcagctct caacccttac
     1861 tacacataaa ataatctgtg ctaaagagaa aatctacaag tgtgaagaat gtggcaaagc
     1921 atttctatgg tcctcaaccc taactagaca taagaggata cacactggag agaaacccta
     1981 caaatgtgaa gaatgtggca aagcttttag ccattcttca acccttgcta aacataagag
     2041 aattcatact ggagagaaac cctacaaatg tgaagaatgt ggcaaagctt ttagccgttc
     2101 ttcaaccctt gctaaacata agagaattca tactggagag aaaccctaca aatgtaaaga
     2161 atgtggcaaa gcttttagca attcctcaac ccttgctaat cataagataa ctcatactga
     2221 agagaaaccc tacaaatgta aagaatgtga caaagctttt aagcgactct caacccttac
     2281 taaacataaa ataatacatg ctggagagaa actctacaaa tgtgaagaat gtggcaaagc
     2341 ttttaatcga tcttcaaatc ttactataca taagtttatt catactggag agaaacctta
     2401 caagtgtgaa gaatgtggca aagcatttaa ctggtcctca agccttacta aacataaaag
     2461 atttcatact agagagaaac ccttcaaatg taaagaatgt ggcaaagcat ttatatggtc
     2521 ttcaacccta actagacata agaggataca cactggagag aagccctaca aatgtgaaga
     2581 atgtggcaaa gcttttaggc aatcctcaac ccttactaaa cataagataa ttcatactgg
     2641 agagaaaccc tacaaatttg aagaatgtgg caaagctttt agacaatcct taacccttaa
     2701 taaacataag ataattcata gtagagagaa accctacaaa tgtaaagaat gtggcaaagc
     2761 ttttaagcaa ttctcaaccc ttactacaca taaaataatt catgctggaa agaaactcta
     2821 caaatgtgaa gaatgtggca aagcttttaa tcattcctca agtctttcta cacataagat
     2881 aattcatact ggagagaagt cttacaagtg tgaagaatgt ggcaaagcat ttctatggtc
     2941 ctcaacccta agaagacata agaggataca cactggagag aaaccctaca aatgtgaaga
     3001 atgtggcaaa gcttttagcc attcttcagc ccttgctaaa cataagagaa ttcatactgg
     3061 agagaaaccc tacaaatgta aagaatgtgg caaagctttt agcaattcct caacccttgc
     3121 taatcataag ataactcata ctgaagagaa accctacaaa tgtaaagaat gtgacaaaac
     3181 ttttaagcga ctctcaaccc ttactaaaca taaaataata catgctggag agaaactcta
     3241 caaatgtgaa gaatgtggca aagcttttaa tcgatcttca aatcttacta tacataagtt
     3301 tattcatact ggagagaaac cttacaagtg tgaagaatgt ggcaaagcat ttaactggtc
     3361 ctcaagcctt actaaacata aaagaattca tactagagag aaacccttca aatgtaaaga
     3421 atgtggcaaa gcatttatat ggtcttcaac cctaactaga cataagagga tacacactgg
     3481 agagaagccc tacaaatgtg aagaatgtgg caaagctttt agccgttcct caacccttac
     3541 taagcataag acaattcata ctggagagaa accctacaaa tgtaaagaat gtggcaaagc
     3601 ttttaagcac tcctcagccc ttgctaaaca taaaataata catgctggag agaaactcta
     3661 caaatgtgag gaatgtggca aagcttttaa tcaatcttca aatcttacga cacataagat
     3721 aattcatact aaagagaaac cttccaagag tgaagaatgt gacaaagcat ttatctggtc
     3781 ctcaaccctt actgaacata agagaattca taccagagag aaaacctaca aatgtgaaga
     3841 atgtggcaaa gcatttagcc agccttcaca ccttactaca cataagagga tgcacactgg
     3901 agagaaaccc tacaaatgtg aagaatgtgg caaagctttt agccaatcct caacccttac
     3961 tacacataag ataattcata ctggagagaa accctacaaa tgtgaagaat gtggcaaagc
     4021 ttttaggaaa tcttcaactc ttactgaaca taagataatt catactggag agaaacccta
     4081 caaatgtgaa gaatgtggca aagcatttag ccaatcctca accctaacta gacatacgag
     4141 gatgcacact ggagagaaac catacaaatg tgaagaatgt ggcaaagctt ttaatcgatc
     4201 ctcaaagctt actacacata agataattca tactggagag aaaccttaca agtgtgaaga
     4261 atgtggcaaa gcatttatat catcctcaac cctaaatgga cataagagaa ttcatactag
     4321 agagaaaccc tacaaatgtg aagaatgtgg caaagcattt agccaatctt caaccctaac
     4381 tagacataag aggttgcaca ccggagagaa accctacaaa tgtggagaat gtggcaaagc
     4441 ctttaaagag tcctcagctc ttactaaaca taagataatt cacactggag agaaacccta
     4501 caaatgtgaa aaatgtggca aagcctttaa ccagtcttca atccttacta accataagaa
     4561 aattcatact atcacacctg taatcccact actttgggag gccgaggcgg gcggatcacg
     4621 aggtcaggag atggagacca tcctggctaa cacagtgaaa ccccttctct actagtaatg
     4681 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     4741 cgagcgttgc ttgcgcgagg acagcaaacg cgttaagtcg acaatcaacc tctggattac
     4801 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4861 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4921 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     4981 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     5041 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     5101 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     5161 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     5221 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     5281 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     5341 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     5401 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5461 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     5521 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5581 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5641 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     5701 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     5761 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5821 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5881 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5941 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6001 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     6061 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     6121 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     6181 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     6241 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6301 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6361 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6421 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6481 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6541 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6601 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6661 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     6721 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6781 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6841 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6901 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6961 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7021 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7081 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7141 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7201 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7261 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7321 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7381 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7441 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7501 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7561 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7621 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7681 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7741 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7801 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7861 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7921 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7981 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     8041 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8101 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8161 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8221 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8281 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8341 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8401 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8461 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8521 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8581 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8641 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8701 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     8761 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     8821 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     8881 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     8941 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     9001 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     9061 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     9121 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     9181 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     9241 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     9301 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     9361 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     9421 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     9481 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     9541 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     9601 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     9661 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     9721 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     9781 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     9841 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     9901 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     9961 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
    10021 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
    10081 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
    10141 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
    10201 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
    10261 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
    10321 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
    10381 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
    10441 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
    10501 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
    10561 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
    10621 cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag
    10681 tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc
    10741 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    10801 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    10861 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
    10921 tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
    10981 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
    11041 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
    11101 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
    11161 cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
    11221 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
    11281 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
    11341 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
    11401 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
    11461 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
    11521 catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa atggcagtat
    11581 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    11641 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    11701 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggcct
    11761 gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
    11821 ctaggtcttg aaaggagtgg gaatt
//
